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PDBsum entry 1elg
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Complex (hydrolase/inhibitor)
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PDB id
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1elg
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References listed in PDB file
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Key reference
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Title
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Nature of the inactivation of elastase by n-Peptidyl-O-Aroyl hydroxylamine as a function of ph.
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Authors
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X.Ding,
B.F.Rasmussen,
H.U.Demuth,
D.Ringe,
A.C.Steinmetz.
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Ref.
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Biochemistry, 1995,
34,
7749-7756.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
perfect match.
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Abstract
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The mechanism of inactivation of porcine pancreatic elastase (PPE) by
N-peptidyl-O-aroylhydroxylamine was studied by X-ray crystallography. The
inactivator forms a stable complex with the enzyme by means of a covalent
attachment to the active site Ser 203(195) O gamma. The nature of the complex
is, however, different depending on the pH at which the inactivation reaction
occurs. At pH 5, the complex formed is a hydroxylamine derivative of Ser
203(195) in which the O gamma of serine is the oxygen of the hydroxylamine
derivative. At pH 7.5, the complex formed is a carbamate derivative at Ser
203(195) O gamma. In both types of complexes, the inactivator binds in the S'
subsites of the enzyme instead of forming the usual antiparallel beta-sheet with
the S subsites. The implication for the mechanism of inactivation at different
pHs is discussed.
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Secondary reference #1
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Title
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Direct structural observation of an acyl-Enzyme intermediate in the hydrolysis of an ester substrate by elastase.
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Authors
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X.Ding,
B.F.Rasmussen,
G.A.Petsko,
D.Ringe.
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Ref.
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Biochemistry, 1994,
33,
9285-9293.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Inhibition of proteases with enkephalin-Analogue inhibitors.
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Authors
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H.U.Demuth,
J.Silberring,
F.Nyberg.
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Ref.
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J Enzyme Inhib, 1991,
4,
289-298.
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PubMed id
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Secondary reference #3
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Title
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Competing redox and inactivation processes in the inhibition of cysteine proteinases by peptidyl o-Acylhydroxamates. 13c and 15n nmr evidence for a novel sulfenamide enzyme adduct
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Authors
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V.J.Robinson,
P.J.Coles,
R.A.Smith,
A.Krantz.
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Ref.
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j am chem soc, 1991,
113,
7760.
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Secondary reference #4
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Title
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N-O bond fission as the rate-Determining step in the aqueous conversion of n-Peptiyl-O-(P-Nitrobenzoyl)hydroxylamines to p-Nitrobenzoic acid and peptidylhydroxamic acids
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Authors
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H.-U.Demuth,
G.Fischer,
A.Barth,
R.L.Schowen.
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Ref.
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j org chem, 1989,
54,
5880.
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Secondary reference #5
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Title
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Crystal structure of the covalent complex formed by a peptidyl difluoro keto amide with porcine pancreatic elastase at 1.78 angstroms resolution
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Authors
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L.H.Takahashi,
R.Radhakrishnan,
R.E.Rosenfield junior,
E.F.Meyer junior,
D.A.Trainor.
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Ref.
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j am chem soc, 1989,
111,
3368.
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