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PDBsum entry 1eik

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Transferase PDB id
1eik
Jmol
Contents
Protein chain
77 a.a. *
* Residue conservation analysis
HEADER    TRANSFERASE                             25-FEB-00   1EIK
TITLE     SOLUTION STRUCTURE OF RNA POLYMERASE SUBUNIT RPB5 FROM
TITLE    2 METHANOBACTERIUM THERMOAUTOTROPHICUM
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: RNA POLYMERASE SUBUNIT RPB5;
COMPND   3 CHAIN: A;
COMPND   4 EC: 2.7.7.6;
COMPND   5 ENGINEERED: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: METHANOTHERMOBACTER
SOURCE   3 THERMAUTOTROPHICUS;
SOURCE   4 ORGANISM_TAXID: 145262;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: PET15B
KEYWDS    RPB5, RPBH, RNA POLYMERASE SUBUNIT, OCSP, NESG, PROTEIN
KEYWDS   2 STRUCTURE INITIATIVE, STRUCTURAL GENOMICS, PSI, NORTHEAST
KEYWDS   3 STRUCTURAL GENOMICS CONSORTIUM, TRANSFERASE
EXPDTA    SOLUTION NMR
NUMMDL    10
AUTHOR    A.YEE,V.BOOTH,A.DHARAMSI,A.ENGEL,A.M.EDWARDS,C.H.ARROWSMITH,
AUTHOR   2 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG)
REVDAT   4   24-FEB-09 1EIK    1       VERSN
REVDAT   3   25-JAN-05 1EIK    1       AUTHOR KEYWDS
REVDAT   2   12-OCT-04 1EIK    1       KEYWDS JRNL   REMARK MASTER
REVDAT   1   21-JUN-00 1EIK    0
JRNL        AUTH   A.YEE,V.BOOTH,A.DHARAMSI,A.ENGEL,A.M.EDWARDS,
JRNL        AUTH 2 C.H.ARROWSMITH
JRNL        TITL   SOLUTION STRUCTURE OF THE RNA POLYMERASE SUBUNIT
JRNL        TITL 2 RPB5 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM.
JRNL        REF    PROC.NATL.ACAD.SCI.USA        V.  97  6311 2000
JRNL        REFN                   ISSN 0027-8424
JRNL        PMID   10841538
JRNL        DOI    10.1073/PNAS.97.12.6311
REMARK   1
REMARK   2
REMARK   2 RESOLUTION. NOT APPLICABLE.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : X-PLOR
REMARK   3   AUTHORS     : BRUNGER
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: NULL
REMARK   4
REMARK   4 1EIK COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 06-MAR-00.
REMARK 100 THE RCSB ID CODE IS RCSB010610.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210  EXPERIMENT TYPE                : NMR
REMARK 210  TEMPERATURE           (KELVIN) : 298
REMARK 210  PH                             : 6.5
REMARK 210  IONIC STRENGTH                 : 150 MM NACL
REMARK 210  PRESSURE                       : 1 ATM
REMARK 210  SAMPLE CONTENTS                : U-15N, 13C; 150 MM NACL, 25
REMARK 210                                   MM PHOSPHATE, 10% D2O, PH 6.5
REMARK 210
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_
REMARK 210                                   15N-SEPARATED_NOESY
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ, 500 MHZ
REMARK 210  SPECTROMETER MODEL             : INOVA
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN
REMARK 210
REMARK 210  STRUCTURE DETERMINATION.
REMARK 210   SOFTWARE USED                 : NMRPIPE, NMRVIEW 3.0
REMARK 210   METHOD USED                   : DISTANCE GEOMETRY  SIMULATED
REMARK 210                                   ANNEALING
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 50
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST
REMARK 210                                   ENERGY
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL
REMARK 210
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR
REMARK 210  SPECTROSCOPY
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500  1 ARG A   3      -67.20    -92.59
REMARK 500  1 LEU A   6       55.67   -169.34
REMARK 500  1 LYS A   7      -74.60     64.29
REMARK 500  1 ASP A  30       48.23     86.94
REMARK 500  1 LEU A  36      166.22    -49.19
REMARK 500  1 PRO A  44      -18.43    -48.17
REMARK 500  1 ILE A  49       26.35     45.45
REMARK 500  1 ARG A  53      100.61    -54.74
REMARK 500  1 THR A  65       44.36     81.25
REMARK 500  2 GLU A   4     -162.65   -100.81
REMARK 500  2 LYS A   7       36.74   -177.42
REMARK 500  2 HIS A   8     -164.96   -175.46
REMARK 500  2 LEU A  10      160.11    -49.93
REMARK 500  2 ASP A  30       44.98     84.95
REMARK 500  2 PRO A  44      -16.98    -48.48
REMARK 500  2 ILE A  49       25.31     45.28
REMARK 500  2 ARG A  53      109.24    -57.35
REMARK 500  2 THR A  65       53.82     82.47
REMARK 500  3 LYS A   2       67.46   -101.86
REMARK 500  3 GLU A   4     -162.41     64.32
REMARK 500  3 ILE A   5      -86.31    -72.09
REMARK 500  3 LEU A   6      106.40     53.29
REMARK 500  3 LYS A   7      162.23     60.66
REMARK 500  3 LEU A  10      -62.50   -102.11
REMARK 500  3 PRO A  12     -177.32    -44.43
REMARK 500  3 ASP A  30       48.12     82.96
REMARK 500  3 PRO A  44      -18.41    -49.02
REMARK 500  3 ILE A  49       25.73     45.50
REMARK 500  3 ARG A  53      100.91    -54.81
REMARK 500  3 THR A  65       45.93     81.46
REMARK 500  3 ALA A  66       27.52     44.41
REMARK 500  4 LYS A   2      -70.03   -111.52
REMARK 500  4 ARG A   3       73.09     56.03
REMARK 500  4 LYS A   7     -158.41   -175.18
REMARK 500  4 HIS A   8       77.49    -63.40
REMARK 500  4 LEU A  10      -84.43     58.17
REMARK 500  4 PRO A  12     -179.13    -65.12
REMARK 500  4 ASP A  30       44.39     83.91
REMARK 500  4 PRO A  44      -19.10    -48.66
REMARK 500  4 ILE A  49       27.02     43.75
REMARK 500  4 ARG A  53       98.08    -54.79
REMARK 500  4 THR A  65      -72.36     85.55
REMARK 500  4 ALA A  66       57.44    174.39
REMARK 500  5 LEU A   6       93.20   -163.25
REMARK 500  5 LYS A   7      -42.03    179.87
REMARK 500  5 LEU A  10      128.99     65.21
REMARK 500  5 ASP A  30       42.51     83.78
REMARK 500  5 LEU A  36      156.14    -47.61
REMARK 500  5 PRO A  44      -19.52    -47.55
REMARK 500  5 ILE A  49       27.58     43.93
REMARK 500  5 ARG A  53       98.64    -54.48
REMARK 500  5 THR A  65     -157.85     76.34
REMARK 500  5 ALA A  66       41.35    -89.92
REMARK 500  6 LYS A   2      -78.01   -103.76
REMARK 500  6 GLU A   4     -167.34   -112.47
REMARK 500  6 LEU A   6       98.02     39.47
REMARK 500  6 LYS A   7       55.16   -165.72
REMARK 500  6 LEU A  10      -65.46   -100.83
REMARK 500  6 ASP A  30       44.45     82.64
REMARK 500  6 PRO A  44      -17.81    -48.43
REMARK 500  6 ILE A  49       25.20     44.90
REMARK 500  6 ARG A  53      100.65    -56.94
REMARK 500  6 THR A  65       44.81     84.49
REMARK 500  7 GLU A   4     -162.38   -120.87
REMARK 500  7 LEU A   6      145.40     71.84
REMARK 500  7 LYS A   7      -46.87   -174.29
REMARK 500  7 GLN A   9       92.26     57.93
REMARK 500  7 ASP A  30       44.60    147.23
REMARK 500  7 PRO A  44      -19.91    -47.71
REMARK 500  7 ILE A  49       25.62     44.31
REMARK 500  7 ARG A  53      100.46    -55.88
REMARK 500  7 THR A  65     -161.93     75.39
REMARK 500  8 GLU A   4     -163.31   -129.50
REMARK 500  8 HIS A   8       41.78   -156.65
REMARK 500  8 LEU A  10      -83.10   -106.48
REMARK 500  8 ASP A  30       33.69     84.92
REMARK 500  8 LEU A  36      157.33    -48.81
REMARK 500  8 PRO A  44      -19.56    -47.74
REMARK 500  8 ILE A  49       27.08     43.85
REMARK 500  8 THR A  65       13.22     81.78
REMARK 500  8 ALA A  66       34.20     75.14
REMARK 500  9 LYS A   2      119.75     64.89
REMARK 500  9 ILE A   5       63.21    -69.78
REMARK 500  9 LEU A   6      146.35    175.66
REMARK 500  9 LYS A   7       94.04   -175.30
REMARK 500  9 HIS A   8       33.73   -148.47
REMARK 500  9 LEU A  10       28.13   -148.82
REMARK 500  9 PRO A  12     -167.51    -77.63
REMARK 500  9 ASP A  30       40.86     83.88
REMARK 500  9 PRO A  44      -18.02    -46.53
REMARK 500  9 ILE A  49       26.76     44.05
REMARK 500  9 ARG A  53      101.78    -54.59
REMARK 500  9 THR A  65       46.66     78.34
REMARK 500  9 ALA A  66       26.76     46.17
REMARK 500 10 LYS A   2      -66.20   -135.84
REMARK 500 10 GLU A   4     -163.45     65.99
REMARK 500 10 LEU A   6      -48.04   -131.77
REMARK 500 10 LYS A   7       48.56   -176.19
REMARK 500 10 GLN A   9       87.85   -165.53
REMARK 500 10 LEU A  10      152.08     59.99
REMARK 500 10 ASP A  30       45.44     84.31
REMARK 500 10 PRO A  44      -19.08    -48.36
REMARK 500 10 ILE A  49       27.66     43.90
REMARK 500 10 ARG A  53       99.38    -54.06
REMARK 500 10 THR A  65     -160.92     77.22
REMARK 500 10 ALA A  66       46.66    -88.91
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: TT9   RELATED DB: TARGETDB
DBREF  1EIK A    1    77  UNP    O27122   RPOH_METTH       1     77
SEQRES   1 A   77  MET LYS ARG GLU ILE LEU LYS HIS GLN LEU VAL PRO GLU
SEQRES   2 A   77  HIS VAL ILE LEU ASN GLU SER GLU ALA LYS ARG VAL LEU
SEQRES   3 A   77  LYS GLU LEU ASP ALA HIS PRO GLU GLN LEU PRO LYS ILE
SEQRES   4 A   77  LYS THR THR ASP PRO VAL ALA LYS ALA ILE GLY ALA LYS
SEQRES   5 A   77  ARG GLY ASP ILE VAL LYS ILE ILE ARG LYS SER PRO THR
SEQRES   6 A   77  ALA GLU GLU PHE VAL THR TYR ARG LEU VAL GLN ASP
HELIX    1   1 ASN A   18  LEU A   29  1                                  12
HELIX    2   2 ASP A   43  ALA A   48  1                                   6
HELIX    3   3 ILE A   49  ALA A   51  5                                   3
SHEET    1   A 3 GLU A  13  LEU A  17  0
SHEET    2   A 3 ILE A  56  SER A  63 -1  O  ILE A  56   N  LEU A  17
SHEET    3   A 3 GLU A  67  LEU A  74 -1  O  GLU A  67   N  SER A  63
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      1.000000  0.000000  0.000000        0.00000
SCALE2      0.000000  1.000000  0.000000        0.00000
SCALE3      0.000000  0.000000  1.000000        0.00000
      
PROCHECK
Go to PROCHECK summary
 References