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UniProt functional annotation for P0A9J8 | ||
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| UniProt code: P0A9J8. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate. {ECO:0000269|PubMed:4261395}. |
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| Catalytic activity: | |
Reaction=chorismate = prephenate; Xref=Rhea:RHEA:13897, ChEBI:CHEBI:29748, ChEBI:CHEBI:29934; EC=5.4.99.5; Evidence={ECO:0000269|PubMed:4261395}; |
| Catalytic activity: | |
Reaction=H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O; Xref=Rhea:RHEA:21648, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:18005, ChEBI:CHEBI:29934; EC=4.2.1.51; Evidence={ECO:0000269|PubMed:4261395}; |
| Activity regulation: | |
Both activities are inhibited by L-phenylalanine. {ECO:0000269|PubMed:4261395}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=45 uM for chorismate (at pH 7.8 and at 37 degrees Celsius) {ECO:0000269|PubMed:4261395}; KM=1.0 mM for prephenate (at pH 7.8 and at 37 degrees Celsius) {ECO:0000269|PubMed:4261395}; KM=147 uM for chorismate (at pH 4.9) {ECO:0000269|Ref.8}; KM=296 uM for chorismate (at pH 7.5) {ECO:0000269|Ref.8}; Note=kcat is 72 sec(-1) at pH 7.5. kcat is 31 sec(-1) at pH 4.9. {ECO:0000269|Ref.8}; pH dependence: Optimum pH is 7.3 for chorismate mutase activity. {ECO:0000269|PubMed:4261395}; |
| Pathway: | |
Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1. {ECO:0000305}. |
| Pathway: | |
Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1. {ECO:0000305}. |
| Subunit: | |
Homodimer. {ECO:0000269|Ref.10}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.