PDBsum entry 1ebp

Complex (cytokine receptor/peptide)

PDB id: 1ebp

Contents

Protein chains

211 a.a. *

16 a.a. *

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: Functional mimicry of a protein hormone by a peptide agonist: the epo receptor complex at 2.8 a.
• Authors: O.Livnah, E.A.Stura, D.L.Johnson, S.A.Middleton, L.S.Mulcahy, N.C.Wrighton, W.J.Dower, L.K.Jolliffe, I.A.Wilson.
• Ref.: Science, 1996, 273, 464-471. [DOI no: 10.1126/science.273.5274.464]
• PubMed id: 8662530

Abstract

The functional mimicry of a protein by an unrelated small molecule has been a formidable challenge. Now, however, the biological activity of a 166-residue hematopoietic growth hormone, erythropoietin (EPO), with its class 1 cytokine receptor has been mimicked by a 20-residue cyclic peptide unrelated in sequence to the natural ligand. The crystal structure at 2.8 A resolution of a complex of this agonist peptide with the extracellular domain of EPO receptor reveals that a peptide dimer induces an almost perfect twofold dimerization of the receptor. The dimer assembly differs from that of the human growth hormone (hGH) receptor complex and suggests that more than one mode of dimerization may be able to induce signal transduction and cell proliferation. The EPO receptor binding site, defined by peptide interaction, corresponds to the smaller functional epitope identified for hGH receptor. Similarly, the EPO mimetic peptide ligand can be considered as a minimal hormone, and suggests the design of nonpeptidic small molecule mimetics for EPO and other cytokines may indeed be achievable.

Secondary reference #1

• Title: Small peptides as potent mimetics of the protein hormone erythropoietin.
• Authors: N.C.Wrighton, F.X.Farrell, R.Chang, A.K.Kashyap, F.P.Barbone, L.S.Mulcahy, D.L.Johnson, R.W.Barrett, L.K.Jolliffe, W.J.Dower.
• Ref.: Science, 1996, 273, 458-464. [DOI no: 10.1126/science.273.5274.458]
• PubMed id: 8662529