UniProt functional annotation for O95628



	UniProt functional annotation for O95628

UniProt code: O95628.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292). Involved in activation of the JAK/STAT pathway (PubMed:11823428, PubMed:22159038). Catalyzes ubiquitination of methylated RBM15 (PubMed:26575292). Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed:29861391). As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy (PubMed:29861391). {ECO:0000269|PubMed:11823428, ECO:0000269|PubMed:22159038, ECO:0000269|PubMed:26575292, ECO:0000269|PubMed:29861391}.

Catalytic activity: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:11823428, ECO:0000269|PubMed:26575292, ECO:0000269|PubMed:29861391};

Pathway: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:26575292}.

Subunit: Interacts with CNOT1 via its C-terminus but does not stably associate with the CCR4-NOT complex (PubMed:11823428). Interacts (via RING domain) with UBE2D2 (PubMed:15001359). Interacts with ABCE1, PINK1 and PELO (PubMed:29861391). {ECO:0000269|PubMed:11823428, ECO:0000269|PubMed:15001359, ECO:0000269|PubMed:29861391}.

Subcellular location: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.

Ptm: Autoubiquitinated. {ECO:0000269|PubMed:11823428, ECO:0000269|PubMed:15001359}.

Sequence caution: Sequence=AAC72963.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=AAC72963.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305}; Sequence=AAD00180.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=BAC11125.1; Type=Erroneous initiation; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292). Involved in activation of the JAK/STAT pathway (PubMed:11823428, PubMed:22159038). Catalyzes ubiquitination of methylated RBM15 (PubMed:26575292). Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed:29861391). As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy (PubMed:29861391). {ECO:0000269\|PubMed:11823428, ECO:0000269\|PubMed:22159038, ECO:0000269\|PubMed:26575292, ECO:0000269\|PubMed:29861391}.


Catalytic activity:		Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269\|PubMed:11823428, ECO:0000269\|PubMed:26575292, ECO:0000269\|PubMed:29861391};
Pathway:		Protein modification; protein ubiquitination. {ECO:0000269\|PubMed:26575292}.
Subunit:		Interacts with CNOT1 via its C-terminus but does not stably associate with the CCR4-NOT complex (PubMed:11823428). Interacts (via RING domain) with UBE2D2 (PubMed:15001359). Interacts with ABCE1, PINK1 and PELO (PubMed:29861391). {ECO:0000269\|PubMed:11823428, ECO:0000269\|PubMed:15001359, ECO:0000269\|PubMed:29861391}.
Subcellular location:		Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
Ptm:		Autoubiquitinated. {ECO:0000269\|PubMed:11823428, ECO:0000269\|PubMed:15001359}.
Sequence caution:		Sequence=AAC72963.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=AAC72963.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305}; Sequence=AAD00180.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=BAC11125.1; Type=Erroneous initiation; Evidence={ECO:0000305};