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PDBsum entry 1dpo
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Serine protease
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PDB id
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1dpo
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References listed in PDB file
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Key reference
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Title
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1.59 a structure of trypsin at 120 k: comparison of low temperature and room temperature structures.
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Authors
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T.Earnest,
E.Fauman,
C.S.Craik,
R.Stroud.
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Ref.
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Proteins, 1991,
10,
171-187.
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PubMed id
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Abstract
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The structure of a rat trypsin mutant [S195C] at a temperature of 120 K has been
refined to a crystallographic R factor of 17.4% between 12.0 and 1.59 A and is
compared with the structure of the D102N mutant at 295 K. A reduction in the
unit cell dimensions in going from room temperature to low temperature is
accompanied by a decrease in molecular surface area and radius of gyration. The
overall structure remains similar to that at room temperature. The attainable
resolution appears to be improved due to the decrease in the fall off of
intensities with resolution [reduction of the temperature factor]. This
decreases the uncertainty in the atomic positions and allows the localization of
more protein atoms and solvent molecules in the low temperature map. The largest
differences between the two models occur at residues with higher than average
temperature factors. Several features can be localized in the solvent region of
the 120 K map that are not seen in the 295 K map. These include several more
water molecules as well as an interstitial sulfate ion and two interstitial
benzamidine molecules.
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Secondary reference #1
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Title
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The three-Dimensional structure of asn102 mutant of trypsin: role of asp102 in serine protease catalysis.
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Authors
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S.Sprang,
T.Standing,
R.J.Fletterick,
R.M.Stroud,
J.Finer-Moore,
N.H.Xuong,
R.Hamlin,
W.J.Rutter,
C.S.Craik.
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Ref.
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Science, 1987,
237,
905-909.
[DOI no: ]
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PubMed id
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