PDBsum entry 1dgu

Blood clotting

PDB id

1dgu

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Contents

			Protein chain

					183 a.a. *

* Residue conservation analysis

PDB id:

1dgu

Links
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Name:

Blood clotting

Title:

Homology-based model of calcium-saturated cib (calcium-and integrin- binding protein)

Structure:

Calcium-saturated cib. Chain: a

Source:

Homo sapiens. Human. Organism_taxid: 9606

NMR struc:

1 models

Authors:

P.M.Hwang,H.J.Vogel

Key ref:

P.M.Hwang and H.J.Vogel (2000). Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies. J Mol Recognit, 13, 83-92. PubMed id: 10822252

Date:

25-Nov-99

Release date:

08-Dec-99

PROCHECK

	Headers

	References

Protein chain

Q99828 (CIB1_HUMAN) - Calcium and integrin-binding protein 1 from Homo sapiens

Seq: Struc:					191 a.a. 183 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	J Mol Recognit 13:83-92 (2000)
PubMed id: 10822252

Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies.
P.M.Hwang, H.J.Vogel.

ABSTRACT

Calcium- and integrin-binding protein (CIB) binds to the 20-residue alphaIIb cytoplasmic domain of platelet alphaIIbbeta3 integrin. Amino acid sequence similarities with calmodulin (CaM) and calcineurin B (CnB) allowed the construction of homology-based models of calcium-saturated CIB as well as apo-CIB. In addition, the solution structure of the alphaIIb cytoplasmic domain in 45% aqueous trifluoroethanol was solved by conventional two-dimensional NMR methods. The models indicate that the N-terminal domain of CIB possesses a number of positively charged residues in its binding site that could interact with the acidic carboxy-terminal LEEDDEEGE sequence of alphaIIb. The C-terminal domain of CIB seems well-suited to bind the sequence WKVGFFKR, which forms a well-structured alpha helix; this is analogous to calmodulin and calcineurin B, which also bind alpha helices. Similarities between the C-terminal domains of CIB and calmodulin suggest that binding of CIB to the cytoplasmic domain of alphaIIb may be affected by fluctuations in the intracellular calcium concentration.

Literature references that cite this PDB file's key reference

PubMed id

Reference

19805025

K.W.Yoon, J.H.Cho, J.K.Lee, Y.H.Kang, J.S.Chae, Y.M.Kim, J.Kim, E.K.Kim, S.E.Kim, J.H.Baik, U.P.Naik, S.G.Cho, and E.J.Choi (2009).
CIB1 functions as a Ca(2+)-sensitive modulator of stress-induced signaling by targeting ASK1.

Proc Natl Acad Sci U S A, 106, 17389-17394.

16825200

A.P.Yamniuk, H.Ishida, and H.J.Vogel (2006).
The interaction between calcium- and integrin-binding protein 1 and the alphaIIb integrin cytoplasmic domain involves a novel C-terminal displacement mechanism.

J Biol Chem, 281, 26455-26464.

15883187

A.P.Yamniuk, and H.J.Vogel (2005).
Calcium- and magnesium-dependent interactions between calcium- and integrin-binding protein and the integrin alphaIIb cytoplasmic domain.

Protein Sci, 14, 1429-1437.

15840829

C.J.Blamey, C.Ceccarelli, U.P.Naik, and B.J.Bahnson (2005).
The crystal structure of calcium- and integrin-binding protein 1: insights into redox regulated functions.

Protein Sci, 14, 1214-1221.

PDB code:

1y1a

15574431

H.R.Gentry, A.U.Singer, L.Betts, C.Yang, J.D.Ferrara, J.Sondek, and L.V.Parise (2005).
Structural and biochemical characterization of CIB1 delineates a new family of EF-hand-containing proteins.

J Biol Chem, 280, 8407-8415.

PDB code:

1xo5

12538886

A.M.Weljie, A.P.Yamniuk, H.Yoshino, Y.Izumi, and H.J.Vogel (2003).
Protein conformational changes studied by diffusion NMR spectroscopy: application to helix-loop-helix calcium binding proteins.

Protein Sci, 12, 228-236.

11983888

A.M.Weljie, P.M.Hwang, and H.J.Vogel (2002).
Solution structures of the cytoplasmic tail complex from platelet integrin alpha IIb- and beta 3-subunits.

Proc Natl Acad Sci U S A, 99, 5878-5883.

PDB codes:

1kup 1kuz

12070332

K.E.Gottschalk, P.D.Adams, A.T.Brunger, and H.Kessler (2002).
Transmembrane signal transduction of the alpha(IIb)beta(3) integrin.

Protein Sci, 11, 1800-1812.

12023286

W.T.Barry, C.Boudignon-Proudhon, D.D.Shock, A.McFadden, J.M.Weiss, J.Sondek, and L.V.Parise (2002).
Molecular basis of CIB binding to the integrin alpha IIb cytoplasmic domain.

J Biol Chem, 277, 28877-28883.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.