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PDBsum entry 1dfb
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Immunoglobulin
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PDB id
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1dfb
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of a human monoclonal antibody FAB fragment against gp41 of human immunodeficiency virus type 1.
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Authors
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X.M.He,
F.Rüker,
E.Casale,
D.C.Carter.
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Ref.
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Proc Natl Acad Sci U S A, 1992,
89,
7154-7158.
[DOI no: ]
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PubMed id
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Abstract
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The three-dimensional structure of a human monoclonal antibody (Fab), which
binds specifically to a major epitope of the transmembrane protein gp41 of the
human immunodeficiency virus type 1, has been determined by crystallographic
methods to a resolution of 2.7 A. It has been previously determined that this
antibody recognizes the epitope SGKLICTTAVPWNAS, belongs to the subclass IgG1
(kappa), and exhibits antibody-dependent cellular cytotoxicity. The quaternary
structure of the Fab is in an extended conformation with an elbow bend angle
between the constant and variable domains of 175 degrees. Structurally, four of
the hypervariable loops can be classified according to previously recognized
canonical structures. The third hypervariable loops of the heavy (H3) and light
chain (L3) are structurally distinct. Hypervariable loop H3, residues 102H-109H,
is unusually extended from the surface. The complementarity-determining region
forms a hydrophobic binding pocket that is created primarily from hypervariable
loops L3, H3, and H2.
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Secondary reference #1
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Title
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Crystallization of the FAB from a human monoclonal antibody against gp 41 of human immunodeficiency virus type i.
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Authors
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E.Casale,
E.Wenisch,
X.M.He,
P.G.Righetti,
R.S.Snyder,
A.Jungbauer,
C.Tauer,
F.Ruker,
D.C.Carter.
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Ref.
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J Mol Biol, 1990,
216,
511-512.
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PubMed id
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