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UniProt functional annotation for P37330 | ||
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| UniProt code: P37330. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl- CoA) and glyoxylate to form malate and CoA. {ECO:0000255|HAMAP- Rule:MF_00641, ECO:0000269|PubMed:14336062, ECO:0000269|PubMed:4892366, ECO:0000269|PubMed:7925370}. |
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| Catalytic activity: | |
Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+); Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.9; Evidence={ECO:0000255|HAMAP- Rule:MF_00641}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00641, ECO:0000269|PubMed:10715138}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=9 uM for acetyl-CoA (at pH 8 and 37 degrees Celsius) {ECO:0000269|PubMed:12930982}; KM=21 uM for glyoxylate (at pH 8 and 37 degrees Celsius) {ECO:0000269|PubMed:12930982}; Vmax=36.1 umol/min/mg enzyme (at pH 8 and 37 degrees Celsius) {ECO:0000269|PubMed:12930982}; Note=Kcat is 48.1 sec(-1) for glyoxylate (at pH 8 and 37 degrees Celsius).; |
| Pathway: | |
Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. {ECO:0000255|HAMAP-Rule:MF_00641}. |
| Subunit: | |
Monomer. {ECO:0000255|HAMAP-Rule:MF_00641, ECO:0000269|PubMed:10715138, ECO:0000269|PubMed:12930982, ECO:0000269|PubMed:15637152, ECO:0000269|PubMed:7925370}. |
| Subcellular location: | |
Cytoplasm. |
| Induction: | |
By glycolate. Part of the glcDEFGB operon, which is induced by growth on glycolate, under the positive control of GlcC. Also induced by growth on acetate. Expression of the glc operon is strongly dependent on the integration host factor (IHF) and is repressed by the global respiratory regulator ArcA-P (PubMed:9880556). {ECO:0000269|PubMed:4892366, ECO:0000269|PubMed:7925370, ECO:0000269|PubMed:9880556}. |
| Disruption phenotype: | |
Cells lacking this gene are unable to oxidize glyoxylate. {ECO:0000269|PubMed:4892366}. |
| Similarity: | |
Belongs to the malate synthase family. GlcB subfamily. {ECO:0000255|HAMAP-Rule:MF_00641}. |
Annotations taken from UniProtKB at the EBI.