PDBsum entry 1clo

Immunoglobulin

PDB id: 1clo

Contents

Protein chains

213 a.a. *

222 a.a. *

Waters ×400

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: Vl:vh domain rotations in engineered antibodies: crystal structures of the FAB fragments from two murine antitumor antibodies and their engineered human constructs.
• Authors: M.J.Banfield, D.J.King, A.Mountain, R.L.Brady.
• Ref.: Proteins, 1997, 29, 161-171.
• PubMed id: 9329081

Abstract

The crystal structures of two pairs of Fab fragments have been determined. The pairs comprise both a murine and an engineered human form, each derived from the antitumor antibodies A5B7 and CTM01. Although antigen specificity is maintained within the pairs, antigen affinity varies. A comparison of the hypervariable loops for each pair of antibodies shows their structure has been well maintained in grafting, supporting the canonical loop model. Detailed structural analysis of the binding sites and domain arrangements for these antibodies suggests the differences in antigen affinity observed are likely to be due to inherent flexibility of the hypervariable loops and movements at the VL:VH domain interface. The four structures provide the first opportunity to study in detail the effects of protein engineering on specific antibodies.

Secondary reference #1

• Title: Vl:vh domain rotations in engineered antibodies: crystal structures of the FAB fragments from two murine antitumor antibodies and their engineered human constructs.
• Authors: M.J.Banfield, D.J.King, A.Mountain, R.L.Brady.
• Ref.: Proteins, 1997, 29, 161-171.
• PubMed id: 9329081

Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a percentage match of 96%.