 |
PDBsum entry 1cfc
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Calcium-binding protein
|
PDB id
|
|
|
|
1cfc
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Solution structure of calcium-Free calmodulin.
|
|
|
Authors
|
|
H.Kuboniwa,
N.Tjandra,
S.Grzesiek,
H.Ren,
C.B.Klee,
A.Bax.
|
|
|
Ref.
|
|
Nat Struct Biol, 1995,
2,
768-776.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The three-dimensional structure of calmodulin in the absence of Ca2+ has been
determined by three- and four-dimensional heteronuclear NMR experiments,
including ROE, isotope-filtering combined with reverse labelling, and
measurement of more than 700 three-bond J-couplings. In analogy with the
Ca(2+)-ligated state of this protein, it consists of two small globular domains
separated by a flexible linker, with no stable, direct contacts between the two
domains. In the absence of Ca2+, the four helices in each of the two globular
domains form a highly twisted bundle, capped by a short anti-parallel
beta-sheet. This arrangement is qualitatively similar to that observed in the
crystal structure of the Ca(2+)-free N-terminal domain of troponin C.
|
|
|
|
|
|
|
