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PDBsum entry 1cfb
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protein ligands metals links
Neural adhesion molecule PDB id
1cfb

 

 

 

 

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Contents
Protein chain
205 a.a. *
Ligands
NAG-NAG
NAG
SO4
Metals
_NA
Waters ×237
* Residue conservation analysis
PDB id:
1cfb
Name: Neural adhesion molecule
Title: Crystal structure of tandem type iii fibronectin domains from drosophila neuroglian at 2.0 angstroms
Structure: Drosophila neuroglian. Chain: a. Engineered: yes
Source: Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Cell_line: s2. Gene: potential
Resolution:
2.00Å     R-factor:   0.202    
Authors: A.H.Huber,Y.E.Wang,A.J.Bieber,P.J.Bjorkman
Key ref: A.H.Huber et al. (1994). Crystal structure of tandem type III fibronectin domains from Drosophila neuroglian at 2.0 A. Neuron, 12, 717-731. PubMed id: 7512815 DOI: 10.1016/0896-6273(94)90326-3
Date:
27-Aug-94     Release date:   30-Nov-94    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
P20241  (NRG_DROME) -  Neuroglian from Drosophila melanogaster
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1302 a.a.
205 a.a.
Key:    Secondary structure  CATH domain

 

 
DOI no: 10.1016/0896-6273(94)90326-3 Neuron 12:717-731 (1994)
PubMed id: 7512815  
 
 
Crystal structure of tandem type III fibronectin domains from Drosophila neuroglian at 2.0 A.
A.H.Huber, Y.M.Wang, A.J.Bieber, P.J.Bjorkman.
 
  ABSTRACT  
 
We report the crystal structure of two adjacent fibronectin type III repeats from the Drosophila neural cell adhesion molecule neuroglian. Each domain consists of two antiparallel beta sheets and is folded topologically identically to single fibronectin type III domains from the extracellular matrix proteins tenascin and fibronectin. beta bulges and left-handed polyproline II helices disrupt the regular beta sheet structure of both neuroglian domains. The hydrophobic interdomain interface includes a metal-binding site, presumably involved in stabilizing the relative orientation between domains and predicted by sequence comparision to be present in the vertebrate homolog molecule L1. The neuroglian domains are related by a near perfect 2-fold screw axis along the longest molecular dimension. Using this relationship, a model for arrays of tandem fibronectin type III repeats in neuroglian and other molecules is proposed.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20971194 F.Yang, A.P.West, and P.J.Bjorkman (2011).
Crystal structure of a hemojuvelin-binding fragment of neogenin at 1.8Å.
  J Struct Biol, 174, 239-244.
PDB code: 3p4l
20969804 X.L.Kuang, X.M.Zhao, H.F.Xu, Y.Y.Shi, J.B.Deng, and G.T.Sun (2010).
Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development.
  BMC Neurosci, 11, 137.  
19845005 A.A.McFarlane, and J.Stetefeld (2009).
An interdomain disulfide bridge links the NtA and first FS domain in agrin.
  Protein Sci, 18, 2421-2428.
PDB code: 3i70
18430740 C.Creze, S.Castang, E.Derivery, R.Haser, N.Hugouvieux-Cotte-Pattat, V.E.Shevchik, and P.Gouet (2008).
The crystal structure of pectate lyase peli from soft rot pathogen Erwinia chrysanthemi in complex with its substrate.
  J Biol Chem, 283, 18260-18268.
PDB codes: 3b4n 3b8y 3b90
18261743 F.Carafoli, J.L.Saffell, and E.Hohenester (2008).
Structure of the tandem fibronectin type 3 domains of neural cell adhesion molecule.
  J Mol Biol, 377, 524-534.
PDB codes: 2vkw 2vkx
16337695 A.M.Craig, E.R.Graf, and M.W.Linhoff (2006).
How to build a central synapse: clues from cell culture.
  Trends Neurosci, 29, 8.  
15803458 K.C.Hwang, S.Y.Park, S.P.Park, J.H.Lim, X.S.Cui, and N.H.Kim (2005).
Specific maternal transcripts in bovine oocytes and cleavaged embryos: identification with novel DDRT-PCR methods.
  Mol Reprod Dev, 71, 275-283.  
16027151 S.S.Mendiratta, N.Sekulic, A.Lavie, and K.J.Colley (2005).
Specific amino acids in the first fibronectin type III repeat of the neural cell adhesion molecule play a role in its recognition and polysialylation by the polysialyltransferase ST8Sia IV/PST.
  J Biol Chem, 280, 32340-32348.  
16061370 Y.Mao, and J.E.Schwarzbauer (2005).
Fibronectin fibrillogenesis, a cell-mediated matrix assembly process.
  Matrix Biol, 24, 389-399.  
15703481 D.Bumbaca, J.E.Littlejohn, H.Nayakanti, D.J.Rigden, M.Y.Galperin, and M.J.Jedrzejas (2004).
Sequence analysis and characterization of a novel fibronectin-binding repeat domain from the surface of Streptococcus pneumoniae.
  OMICS, 8, 341-356.  
15213410 E.Rudiño-Piñera, U.Schwarz-Linek, J.R.Potts, and E.F.Garman (2004).
Twinned or not twinned, that is the question: crystallization and preliminary crystallographic analysis of the 2F1(3)F1 module pair of human fibronectin.
  Acta Crystallogr D Biol Crystallogr, 60, 1341-1345.  
14993704 N.Kulahin, C.Kasper, M.Gajhede, V.Berezin, E.Bock, and J.S.Kastrup (2004).
Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1.
  Acta Crystallogr D Biol Crystallogr, 60, 591-593.  
12791681 B.E.Close, S.S.Mendiratta, K.M.Geiger, L.J.Broom, L.L.Ho, and K.J.Colley (2003).
The minimal structural domains required for neural cell adhesion molecule polysialylation by PST/ST8Sia IV and STX/ST8Sia II.
  J Biol Chem, 278, 30796-30805.  
12390682 B.E.Willcox, L.M.Thomas, T.L.Chapman, A.P.Heikema, A.P.West, and P.J.Bjorkman (2002).
Crystal structure of LIR-2 (ILT4) at 1.8 A: differences from LIR-1 (ILT2) in regions implicated in the binding of the Human Cytomegalovirus class I MHC homolog UL18.
  BMC Struct Biol, 2, 6.
PDB code: 2gw5
11733523 B.Kunz, R.Lierheimer, C.Rader, M.Spirig, U.Ziegler, and P.Sonderegger (2002).
Axonin-1/TAG-1 mediates cell-cell adhesion by a cis-assisted trans-interaction.
  J Biol Chem, 277, 4551-4557.  
12601142 C.F.van der Walle, H.Altroff, and H.J.Mardon (2002).
Novel mutant human fibronectin FIII9-10 domain pair with increased conformational stability and biological activity.
  Protein Eng, 15, 1021-1024.  
11959962 G.Baneyx, L.Baugh, and V.Vogel (2002).
Fibronectin extension and unfolding within cell matrix fibrils controlled by cytoskeletal tension.
  Proc Natl Acad Sci U S A, 99, 5139-5143.  
12200278 I.A.Kataeva, R.D.Seidel, A.Shah, L.T.West, X.L.Li, and L.G.Ljungdahl (2002).
The fibronectin type 3-like repeat from the Clostridium thermocellum cellobiohydrolase CbhA promotes hydrolysis of cellulose by modifying its surface.
  Appl Environ Microbiol, 68, 4292-4300.  
11391001 B.E.Gewurz, R.Gaudet, D.Tortorella, E.W.Wang, H.L.Ploegh, and D.C.Wiley (2001).
Antigen presentation subverted: Structure of the human cytomegalovirus protein US2 bound to the class I molecule HLA-A2.
  Proc Natl Acad Sci U S A, 98, 6794-6799.
PDB code: 1im3
10958670 A.C.Wilson, M.Boutros, K.M.Johnson, and W.Herr (2000).
HCF-1 amino- and carboxy-terminal subunit association through two separate sets of interaction modules: involvement of fibronectin type 3 repeats.
  Mol Cell Biol, 20, 6721-6730.  
10811834 D.Fitzli, E.T.Stoeckli, S.Kunz, K.Siribour, C.Rader, B.Kunz, S.V.Kozlov, A.Buchstaller, R.P.Lane, D.M.Suter, W.J.Dreyer, and P.Sonderegger (2000).
A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons.
  J Cell Biol, 149, 951-968.  
  10933504 I.Callebaut, D.Gilgès, I.Vigon, and J.P.Mornon (2000).
HYR, an extracellular module involved in cellular adhesion and related to the immunoglobulin-like fold.
  Protein Sci, 9, 1382-1390.  
10835339 J.Bravo, and J.K.Heath (2000).
Receptor recognition by gp130 cytokines.
  EMBO J, 19, 2399-2411.  
11114384 T.L.Chapman, A.P.Heikema, A.P.West, and P.J.Bjorkman (2000).
Crystal structure and ligand binding properties of the D1D2 region of the inhibitory receptor LIR-1 (ILT2).
  Immunity, 13, 727-736.
PDB code: 1g0x
10216309 D.Bisig, P.Weber, L.Vaughan, K.H.Winterhalter, and K.Piontek (1999).
Purification, crystallization and preliminary crystallographic studies of a two fibronectin type-III domain segment from chicken tenascin encompassing the heparin- and contactin-binding regions.
  Acta Crystallogr D Biol Crystallogr, 55, 1069-1073.
PDB code: 1qr4
10026225 P.Lin, K.Luby-Phelps, and J.T.Stull (1999).
Properties of filament-bound myosin light chain kinase.
  J Biol Chem, 274, 5987-5994.  
10364233 Y.F.Liao, K.G.Wieder, J.M.Classen, and L.Van De Water (1999).
Identification of two amino acids within the EIIIA (ED-A) segment of fibronectin constituting the epitope for two function-blocking monoclonal antibodies.
  J Biol Chem, 274, 17876-17884.  
9782056 C.M.Goll, A.Pastore, and M.Nilges (1998).
The three-dimensional structure of a type I module from titin: a prototype of intracellular fibronectin type III domains.
  Structure, 6, 1291-1302.
PDB code: 1bpv
  9568895 D.K.Smith, and H.R.Treutlein (1998).
LIF receptor-gp130 interaction investigated by homology modeling: implications for LIF binding.
  Protein Sci, 7, 886-896.  
9852159 S.Kunz, M.Spirig, C.Ginsburg, A.Buchstaller, P.Berger, R.Lanz, C.Rader, L.Vogt, B.Kunz, and P.Sonderegger (1998).
Neurite fasciculation mediated by complexes of axonin-1 and Ng cell adhesion molecule.
  J Cell Biol, 143, 1673-1690.  
9868364 T.D.Mulhern, G.W.Booker, and L.Cosgrove (1998).
A third fibronectin-type-III domain in the insulin-family receptors.
  Trends Biochem Sci, 23, 465-466.  
  9605315 Y.A.Muller, R.F.Kelley, and A.M.de Vos (1998).
Hinge bending within the cytokine receptor superfamily revealed by the 2.4 A crystal structure of the extracellular domain of rabbit tissue factor.
  Protein Sci, 7, 1106-1115.
PDB code: 1a21
9223190 A.Romero, P.F.Varela, E.Töpfer-Petersen, and J.J.Calvete (1997).
Crystallization and preliminary X-ray diffraction analysis of bovine seminal plasma PDC-109, a protein composed of two fibronectin type II domains.
  Proteins, 28, 454-456.  
9242926 C.Chothia, and E.Y.Jones (1997).
The molecular structure of cell adhesion molecules.
  Annu Rev Biochem, 66, 823-862.  
9442878 D.J.Leahy (1997).
Implications of atomic-resolution structures for cell adhesion.
  Annu Rev Cell Dev Biol, 13, 363-393.  
9442880 F.S.Walsh, and P.Doherty (1997).
Neural cell adhesion molecules of the immunoglobulin superfamily: role in axon growth and guidance.
  Annu Rev Cell Dev Biol, 13, 425-456.  
8999851 K.C.Ingham, S.A.Brew, S.Huff, and S.V.Litvinovich (1997).
Cryptic self-association sites in type III modules of fibronectin.
  J Biol Chem, 272, 1718-1724.  
9314547 R.Manabe, N.Ohe, T.Maeda, T.Fukuda, and K.Sekiguchi (1997).
Modulation of cell-adhesive activity of fibronectin by the alternatively spliced EDA segment.
  J Cell Biol, 139, 295-307.  
8994808 A.Bateman, and C.Chothia (1996).
Fibronectin type III domains in yeast detected by a hidden Markov model.
  Curr Biol, 6, 1544-1547.  
  8947027 A.Bateman, M.Jouet, J.MacFarlane, J.S.Du, S.Kenwrick, and C.Chothia (1996).
Outline structure of the human L1 cell adhesion molecule and the sites where mutations cause neurological disorders.
  EMBO J, 15, 6050-6059.  
  8880920 A.Lombardo, Y.Wang, C.Z.Ni, X.Dai, C.D.Dickinson, R.Kodandapani, S.Chiang, C.A.White, F.Pio, N.H.Xuong, R.C.Hamlin, E.Ruoslahti, and K.R.Ely (1996).
Conformational flexibility and crystallization of tandemly linked type III modules of human fibronectin.
  Protein Sci, 5, 1934-1938.  
8996796 B.Drescher, E.Spiess, M.Schachner, and R.Probstmeier (1996).
Structural analysis of the murine cell adhesion molecule L1 by electron microscopy and computer-assisted modelling.
  Eur J Neurosci, 8, 2467-2478.  
8548820 D.J.Leahy, I.Aukhil, and H.P.Erickson (1996).
2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region.
  Cell, 84, 155-164.
PDB code: 1fnf
8939654 E.Y.Jones (1996).
Three-dimensional structure of cell adhesion molecules.
  Curr Opin Cell Biol, 8, 602-608.  
8968582 I.Favre, E.Moczydlowski, and L.Schild (1996).
On the structural basis for ionic selectivity among Na+, K+, and Ca2+ in the voltage-gated sodium channel.
  Biophys J, 71, 3110-3125.  
8981327 J.R.Potts, and I.D.Campbell (1996).
Structure and function of fibronectin modules.
  Matrix Biol, 15, 313.  
8870072 P.Bork, A.K.Downing, B.Kieffer, and I.D.Campbell (1996).
Structure and distribution of modules in extracellular proteins.
  Q Rev Biophys, 29, 119-167.  
8805538 S.Improta, A.S.Politou, and A.Pastore (1996).
Immunoglobulin-like modules from titin I-band: extensible components of muscle elasticity.
  Structure, 4, 323-337.
PDB codes: 1tit 1tiu
7777489 A.Gustchina, A.Zdanov, C.Schalk-Hihi, and A.Wlodawer (1995).
A model of the complex between interleukin-4 and its receptors.
  Proteins, 21, 140-148.
PDB code: 1ite
  7737120 I.M.Frick, K.L.Crossin, G.M.Edelman, and L.Björck (1995).
Protein H--a bacterial surface protein with affinity for both immunoglobulin and fibronectin type III domains.
  EMBO J, 14, 1674-1679.  
7755987 T.C.Keller (1995).
Structure and function of titin and nebulin.
  Curr Opin Cell Biol, 7, 32-38.  
7629186 T.F.Busby, W.S.Argraves, S.A.Brew, I.Pechik, G.L.Gilliland, and K.C.Ingham (1995).
Heparin binding by fibronectin module III-13 involves six discontinuous basic residues brought together to form a cationic cradle.
  J Biol Chem, 270, 18558-18562.  
7615642 U.Nörenberg, M.Hubert, T.Brümmendorf, A.Tárnok, and F.G.Rathjen (1995).
Characterization of functional domains of the tenascin-R (restrictin) polypeptide: cell attachment site, binding with F11, and enhancement of F11-mediated neurite outgrowth by tenascin-R.
  J Cell Biol, 130, 473-484.  
9383409 Y.S.Wu, V.L.Bevilacqua, and J.M.Berg (1995).
Fibrillin domain folding and calcium binding: significance to Marfan syndrome.
  Chem Biol, 2, 91-97.  
  7849586 A.A.Kossiakoff, W.Somers, M.Ultsch, K.Andow, Y.A.Muller, and A.M.De Vos (1994).
Comparison of the intermediate complexes of human growth hormone bound to the human growth hormone and prolactin receptors.
  Protein Sci, 3, 1697-1705.  
7712288 G.Wagner, and D.F.Wyss (1994).
Cell surface adhesion receptors.
  Curr Opin Struct Biol, 4, 841-851.  
7937847 H.P.Erickson (1994).
Reversible unfolding of fibronectin type III and immunoglobulin domains provides the structural basis for stretch and elasticity of titin and fibronectin.
  Proc Natl Acad Sci U S A, 91, 10114-10118.  
8081748 I.D.Campbell, and C.Spitzfaden (1994).
Building proteins with fibronectin type III modules.
  Structure, 2, 333-337.  
  7849602 S.E.Brenner, and A.Berry (1994).
A quantitative methodology for the de novo design of proteins.
  Protein Sci, 3, 1871-1882.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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