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PDBsum entry 1c7v
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Metal binding protein
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PDB id
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1c7v
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Contents |
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* Residue conservation analysis
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DOI no:
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Biochemistry
39:7920-7926
(2000)
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PubMed id:
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Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement.
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I.Théret,
S.Baladi,
J.A.Cox,
H.Sakamoto,
C.T.Craescu.
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ABSTRACT
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Calcium vector protein (CaVP) from amphioxus is a two-domain, calcium-binding
protein (18.3 kDa) of the calmodulin superfamily. Only two of the four EF-hand
motifs (sites III and IV) have a significant binding affinity for calcium ions.
We determined the solution structure of the domain containing these active sites
(C-CaVP: W81-S161), in the Ca(2+)-saturated state, using NMR spectroscopy and
restrained molecular dynamics. The tertiary structure is similar to other
Ca(2+)-binding domains containing a pair of EF-hand motifs. The apo state has
spectroscopic and thermodynamic characteristics of a molten globule, with
conserved secondary structure but highly fluctuating tertiary organization.
Titration of C-CaVP with Ca(2+) revealed a stepwise ion binding, with a stable
equilibrium intermediate in which only site III binds a calcium ion. Despite a
highly fluctuating structure of the free site IV, the calcium-bound site III has
a persistent structure, with similar secondary elements but different interhelix
angle and hydrophobic packing relative to the fully calcium-saturated state.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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E.Babini,
I.Bertini,
F.Capozzi,
E.Chirivino,
and
C.Luchinat
(2006).
A structural and dynamic characterization of the EF-hand protein CLSP.
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Structure,
14,
1029-1038.
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PDB code:
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T.L.Wingo,
V.N.Shah,
M.E.Anderson,
T.P.Lybrand,
W.J.Chazin,
and
J.R.Balser
(2004).
An EF-hand in the sodium channel couples intracellular calcium to cardiac excitability.
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Nat Struct Mol Biol,
11,
219-225.
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H.Aitio,
T.Laakso,
T.Pihlajamaa,
M.Torkkeli,
I.Kilpeläinen,
T.Drakenberg,
R.Serimaa,
and
A.Annila
(2001).
Characterization of apo and partially saturated states of calerythrin, an EF-hand protein from S. erythraea: a molten globule when deprived of Ca(2+).
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Protein Sci,
10,
74-82.
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I.Théret,
J.A.Cox,
J.Mispelter,
and
C.T.Craescu
(2001).
Backbone dynamics of the regulatory domain of calcium vector protein, studied by (15)N relaxation at four fields, reveals unique mobility characteristics of the intermotif linker.
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Protein Sci,
10,
1393-1402.
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S.Baladi,
P.O.Tsvetkov,
T.V.Petrova,
T.Takagi,
H.Sakamoto,
V.M.Lobachov,
A.A.Makarov,
and
J.A.Cox
(2001).
Folding units in calcium vector protein of amphioxus: Structural and functional properties of its amino- and carboxy-terminal halves.
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Protein Sci,
10,
771-778.
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D.Kraus,
S.Khoury,
A.Fendyur,
S.G.Kachalsky,
T.Abu-Hatoum,
and
R.Rahamimoff
(2000).
Intracellular calcium dynamics--sparks of insight.
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J Basic Clin Physiol Pharmacol,
11,
331-365.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
