PDBsum entry 1c5a

Complement factor

PDB id: 1c5a

Contents

Protein chain

66 a.a.

References listed in PDB file

Key reference

• Title: Three-Dimensional structure of porcine c5adesarg from 1h nuclear magnetic resonance data.
• Authors: M.P.Williamson, V.S.Madison.
• Ref.: Biochemistry, 1990, 29, 2895-2905. [DOI no: 10.1021/bi00464a002]
• PubMed id: 2337573

Abstract

Two-dimensional nuclear magnetic resonance spectra of porcine C5adesArg (73 residues) have been used to construct a list of 34 hydrogen bonds, 27 dihedral angle constraints, and 151 distance constraints, derived from nuclear Overhauser effect data. These constraints were used in restrained molecular dynamics calculations on residues 1-65 of C5a, starting from a folded structure modeled on the crystal structure of a homologous protein, C3a. Forty-one structures have been calculated, which fall into three similar families with few violations of the imposed constraints. Structures in the most populated family have a root-mean-square deviation from the average structure of 1.02 A for the C alpha atoms, with good definition of the internal residues. There is good agreement between the calculated structures and other nuclear magnetic resonance data. The structure is very similar to that recently reported for human C5a [Zuiderweg et al. (1989) Biochemistry 28, 172-185]. Some biological implications of these structures are discussed.

Secondary reference #1

• Title: 1h nuclear magnetic resonance assignments and secondary structure of porcine c5ades arg.
• Author: M.P.Williamson.
• Ref.: J Mol Biol, 1989, 206, 407-410.
• PubMed id: 2716054