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PDBsum entry 1c4u

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protein ligands metals Protein-protein interface(s) links
Hydrolase/hydrolase inhibitor PDB id
1c4u
Jmol PyMol
Contents
Protein chains
30 a.a. *
252 a.a. *
Ligands
PHE-GLU-GLY-ILE-
PRO-GLY-GLU-TYS
IH1
Metals
_NA
Waters ×95
* Residue conservation analysis
PDB id:
1c4u
Name: Hydrolase/hydrolase inhibitor
Title: Selective non electrophilic thrombin inhibitors with cyclohe moieties.
Structure: Thrombin. Chain: 1. Synonym: coagulation factor ii. Thrombin. Chain: 2. Synonym: coagulation factor ii. Protein (hirugen). Chain: 3. Engineered: yes.
Source: Homo sapiens. Human. Organism_taxid: 9606. Tissue: blood. Synthetic: yes. Other_details: chemically synthesized
Biol. unit: Trimer (from PQS)
Resolution:
2.10Å     R-factor:   0.167    
Authors: R.Krishnan,I.Mochalkin,R.K.Arni,A.Tulinsky
Key ref:
R.Krishnan et al. (2000). Structure of thrombin complexed with selective non-electrophilic inhibitors having cyclohexyl moieties at P1. Acta Crystallogr D Biol Crystallogr, 56, 294-303. PubMed id: 10713516 DOI: 10.1107/S0907444900000068
Date:
25-Sep-99     Release date:   27-Sep-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00734  (THRB_HUMAN) -  Prothrombin
Seq:
Struc:
 
Seq:
Struc:
622 a.a.
30 a.a.
Protein chain
Pfam   ArchSchema ?
P00734  (THRB_HUMAN) -  Prothrombin
Seq:
Struc:
 
Seq:
Struc:
622 a.a.
252 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     blood coagulation   2 terms 
  Biochemical function     calcium ion binding     2 terms  

 

 
DOI no: 10.1107/S0907444900000068 Acta Crystallogr D Biol Crystallogr 56:294-303 (2000)
PubMed id: 10713516  
 
 
Structure of thrombin complexed with selective non-electrophilic inhibitors having cyclohexyl moieties at P1.
R.Krishnan, I.Mochalkin, R.Arni, A.Tulinsky.
 
  ABSTRACT  
 
The crystal structures of five new non-electrophilic beta-strand-templated thrombin active-site inhibitors have been determined bound to the enzyme. Four co-crystallize with hirugen and inhibitor isomorphously to produce thrombin-hirugen crystals (monoclinic, space group C2), while one co-crystallizes in the hexagonal system, space group P6(5). A 1,4-substituted cyclohexyl moiety is conserved at the P1 position of all the inhibitors, along with a fused hetero-bicyclic five- and six-membered ring that occupies the P2 site. Amino, amidino and aminoimidazole groups are attached to the cyclohexyl ring for recognition at the S1 specificity site, while benzylsulfonyl and diphenyl groups enhance the binding at the S3 subsite. The cyclohexyl groups at the P1 positions of three of the inhibitors appear to be in the energetically favored chair conformation, while the imidazole-substituted cyclohexyl rings are in a boat conformation. Somewhat unexpectedly, the two cyclohexyl-aminoimidazole groups bind differently in the specificity site; the unique binding of one is heretofore unreported. The other inhibitors generally mimic arginyl binding at S1. This group of inhibitors combines the non-electrophilicity and selectivity of DAPA-like compounds and the more optimal binding features of the S1-S3 sites of thrombin for peptidic molecules, which results in highly potent (binding constants 12 nM-16 pM, one being 1.1 microM) and selective (ranging from 140 to 20 000 times more selective compared with trypsin) inhibitors of thrombin. The binding modes of these novel inhibitors are correlated with their binding constants, as is their selectivity, in order to provide further insight for the design of therapeutic antithrombotic agents that inhibit thrombin directly at the active site.
 
  Selected figure(s)  
 
Figure 4.
Figure 4 Stereoview of superposed MOL356 and MOL592 bound to thrombin. MOL356 (red), MOL592 (blue); thrombin of MOL356 complex numbered in atom colors; broken lines, principal hydrogen bonds.
Figure 5.
Figure 5 Schematic of hydrogen-bonded salt bridges of Molecumetics inhibitors with Asp189 of the S1 specificity site of thrombin. MOL354 and MOL1245 shown together in one schematic; values for MOL1245 are in parentheses.
 
  The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2000, 56, 294-303) copyright 2000.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
16344948 S.Cotesta, and M.Stahl (2006).
The environment of amide groups in protein-ligand complexes: H-bonds and beyond.
  J Mol Model, 12, 436-444.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.

 

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