UniProt functional annotation for P00915

UniProt code: P00915.

Organism: Homo sapiens (Human).
Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Function: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. {ECO:0000269|PubMed:10550681}.
Catalytic activity: H(2)CO(3) = CO(2) + H(2)O.
Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Enzyme regulation: Activated by histamine, imidazole, L- adrenaline, L- and D-histidine, and L- and D-phenylalanine. Inhibited by coumarins, sulfonamide derivatives such as acetazolamide, benzenesulfonamide and derivatives (4- carboxyethylbenzene-sulfonamide, 4-carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2-aminoethyl)benzene-sulfonamide, 4- aminoethylbenzene-sulfonamide), and 'prong inhibitors' BR15, BR17, BR22 and BR30. Activated by a short exposition to Foscarnet (phosphonoformate trisodium salt), but inhibited by a long one. Esterase activity weakly reduced by cyanamide. {ECO:0000269|PubMed:16506782, ECO:0000269|PubMed:16686544, ECO:0000269|PubMed:16807956, ECO:0000269|PubMed:17127057, ECO:0000269|PubMed:17314045, ECO:0000269|PubMed:17407288, ECO:0000269|PubMed:18618712, ECO:0000269|PubMed:19186056, ECO:0000269|PubMed:19206230}.
Biophysicochemical properties: Kinetic parameters: KM=4.0 mM for CO(2) {ECO:0000269|PubMed:10550681, ECO:0000269|PubMed:18618712}; KM=15 mM for 4-nitrophenyl acetate {ECO:0000269|PubMed:10550681, ECO:0000269|PubMed:18618712};
Subcellular location: Cytoplasm.
Similarity: Belongs to the alpha-carbonic anhydrase family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.