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UniProt functional annotation for P56653 | ||
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| UniProt code: P56653. |
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| Organism: | |
Pseudomonas sp. (strain CBS-3). |
| Taxonomy: | |
Bacteria; Proteobacteria. |
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| Function: | |
Hydrolyzes 4-hydroxybenzoate-CoA, and to a lesser extent benzoyl-CoA and 4-chlorobenzoate-CoA. Not active against aliphatic acyl-CoA thioesters, including palmitoyl-CoA, hexanoyl-CoA and acetyl- CoA. {ECO:0000269|PubMed:1610806}. |
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| Catalytic activity: | |
Reaction=4-hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA + H(+); Xref=Rhea:RHEA:11948, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17879, ChEBI:CHEBI:57287, ChEBI:CHEBI:57356; EC=3.1.2.23; Evidence={ECO:0000255|PROSITE-ProRule:PRU10041, ECO:0000269|PubMed:1610806}; |
| Activity regulation: | |
Unaffected by EDTA, Mg(2+), Mn(2+), Fe(2+), Ca(2+), Co(2+) and Zn(2+). {ECO:0000269|PubMed:1610806}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=6 uM for 4-hydroxybenzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=550 uM for 4-chlorobenzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=200 uM for benzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=270 uM for 4-hydroxybenzoyl-pantetheine {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=56 uM for 4-methoxybenzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=230 uM for 4-methylbenzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=510 uM for benzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=520 uM for 4-fluorobenzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; KM=300 uM for 4-trifluorobenzoyl-CoA {ECO:0000269|PubMed:12220180, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:16962159}; |
| Pathway: | |
Xenobiotic degradation; 4-chlorobenzoate degradation; 4- hydroxybenzoate from 4-chlorobenzoate: step 3/3. {ECO:0000269|PubMed:1610806}. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:11997398, ECO:0000269|PubMed:1610806, ECO:0000269|PubMed:9837940}. |
| Similarity: | |
Belongs to the 4-hydroxybenzoyl-CoA thioesterase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.