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PDBsum entry 1bql
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Antibody/antigen
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PDB id
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1bql
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Contents |
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212 a.a.
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214 a.a.
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129 a.a.
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Refined structures of bobwhite quail lysozyme uncomplexed and complexed with the hyhel-5 FAB fragment.
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Authors
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S.Chacko,
E.W.Silverton,
S.J.Smith-Gill,
D.R.Davies,
K.A.Shick,
K.A.Xavier,
R.C.Willson,
P.D.Jeffrey,
C.Y.Chang,
L.C.Sieker,
S.Sheriff.
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Ref.
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Proteins, 1996,
26,
55-65.
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PubMed id
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Abstract
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The HyHEL-5 antibody has more than a thousandfold lower affinity for bobwhite
quail lysozyme (BWQL) than for hen egg-white lysozyme (HEL). Four sequence
differences exist between BWQL and HEL, of which only one is involved in the
interface with the Fab. The structure of bobwhite quail lysozyme has been
determined in the uncomplexed state in two different crystal forms and in the
complexed state with HyHEL-5, an antihen egg-white lysozyme Fab. Similar
backbone conformations are observed in the three molecules of the two crystal
forms of uncomplexed BWQL, although they show considerable variability in
side-chain conformation. A relatively mobile segment in uncomplexed BWQL is
observed to be part of the HyHEL-5 epitope. No major backbone conformational
differences are observed in the lysozyme upon complex formation, but side-chain
conformational differences are seen in surface residues that are involved in the
interface with the antibody. The hydrogen bonding in the interface between BWQL
and HyHEL-5 is similar to that in previously determined lysozyme-HyHEL-5
complexes.
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Secondary reference #1
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Title
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Structure of an antibody-Lysozyme complex unexpected effect of conservative mutation.
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Authors
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S.Chacko,
E.Silverton,
L.Kam-Morgan,
S.Smith-Gill,
G.Cohen,
D.Davies.
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Ref.
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J Mol Biol, 1995,
245,
261-274.
[DOI no: ]
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PubMed id
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Figure 8.
Figure 8. Hydrogen bonding interactions around the 68(Y) pocket in the native and mutant structures.
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Figure 9.
Figure 9. Hydrogen bonding in the active site of the native and mutant BPTI--trypsin complexes.
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The above figures are
reproduced from the cited reference
with permission from Elsevier
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Secondary reference #2
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Title
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Three-Dimensional structure of an antibody-Antigen complex.
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Authors
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S.Sheriff,
E.W.Silverton,
E.A.Padlan,
G.H.Cohen,
S.J.Smith-Gill,
B.C.Finzel,
D.R.Davies.
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Ref.
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Proc Natl Acad Sci U S A, 1987,
84,
8075-8079.
[DOI no: ]
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PubMed id
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