UniProt functional annotation for Q02821



	UniProt functional annotation for Q02821

UniProt code: Q02821.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.

Function: Functions in nuclear protein import as an adapter protein for importin beta nuclear receptors (PubMed:10913188). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed:10745017). Promotes docking of import substrates to the nuclear envelope (PubMed:8521485). Together with importin beta KAP95, mediates nuclear import of transcription factor GCN4 (PubMed:14648200). Together with tethering factor STS1, targets the proteasome to the nucleus (PubMed:10913188, PubMed:21075847). {ECO:0000269|PubMed:10745017, ECO:0000269|PubMed:10913188, ECO:0000269|PubMed:14648200, ECO:0000269|PubMed:21075847, ECO:0000269|PubMed:8521485}.

Subunit: Forms a complex with an importin beta subunit. In the nucleus, interacts with NUP2 which accelerate release of NLSs, NUP2 is subsequently displaced by CSE1:RanGTP which mediates re-export and recycling. Interacts with HEH2, SHE2, and STS1. {ECO:0000269|PubMed:10745017, ECO:0000269|PubMed:10913188, ECO:0000269|PubMed:14532109, ECO:0000269|PubMed:15602554, ECO:0000269|PubMed:16929305, ECO:0000269|PubMed:19244342, ECO:0000269|PubMed:21075847}.

Subcellular location: Cytoplasm, perinuclear region. Note=Mainly localized at the periphery of the nucleus.

Domain: The NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.

Miscellaneous: Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the karyopherin heterodimer by binding to the beta subunit and releases both subunits from the docking site.

Miscellaneous: Present with 2790 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.

Similarity: Belongs to the importin alpha family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.



Function:		Functions in nuclear protein import as an adapter protein for importin beta nuclear receptors (PubMed:10913188). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed:10745017). Promotes docking of import substrates to the nuclear envelope (PubMed:8521485). Together with importin beta KAP95, mediates nuclear import of transcription factor GCN4 (PubMed:14648200). Together with tethering factor STS1, targets the proteasome to the nucleus (PubMed:10913188, PubMed:21075847). {ECO:0000269\|PubMed:10745017, ECO:0000269\|PubMed:10913188, ECO:0000269\|PubMed:14648200, ECO:0000269\|PubMed:21075847, ECO:0000269\|PubMed:8521485}.


Subunit:		Forms a complex with an importin beta subunit. In the nucleus, interacts with NUP2 which accelerate release of NLSs, NUP2 is subsequently displaced by CSE1:RanGTP which mediates re-export and recycling. Interacts with HEH2, SHE2, and STS1. {ECO:0000269\|PubMed:10745017, ECO:0000269\|PubMed:10913188, ECO:0000269\|PubMed:14532109, ECO:0000269\|PubMed:15602554, ECO:0000269\|PubMed:16929305, ECO:0000269\|PubMed:19244342, ECO:0000269\|PubMed:21075847}.
Subcellular location:		Cytoplasm, perinuclear region. Note=Mainly localized at the periphery of the nucleus.
Domain:		The NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
Miscellaneous:		Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the karyopherin heterodimer by binding to the beta subunit and releases both subunits from the docking site.
Miscellaneous:		Present with 2790 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity:		Belongs to the importin alpha family. {ECO:0000305}.