PDBsum entry 1bg1

Transcription/DNA

PDB id: 1bg1

Contents

Protein chain

559 a.a. *

DNA/RNA

Waters ×154

* Residue conservation analysis

PDB id:

1bg1

Name:

Transcription/DNA

Title:

Transcription factor stat3b/DNA complex

Structure:

DNA (5'- d( Tp Gp Cp Ap Tp Tp Tp Cp Cp Cp Gp Tp Ap Ap Ap Tp Cp T)-3'). Chain: b. Engineered: yes. Other_details: 18-mer chemically synthesized, active form is 17-mer duplex with overhanging ends. Protein (transcription factor stat3b). Chain: a. Engineered: yes

Source:

Synthetic: yes. Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.

Biol. unit:

Tetramer (from PDB file)

Resolution:

2.25Å R-factor: 0.246 R-free: 0.301

Authors:

S.Becker,B.Groner,C.W.Muller

Key ref:

S.Becker et al. (1998). Three-dimensional structure of the Stat3beta homodimer bound to DNA. Nature, 394, 145-151. PubMed id: 9671298 DOI: 10.1038/28101

Date:

03-Jun-98 Release date: 13-Jan-99

Protein chain

P42227  (STAT3_MOUSE) -  Signal transducer and activator of transcription 3 from Mus musculus

Seq: 770 a.a.

Struc: 559 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chain

T-G-C-A-T-T-T-C-C-C-G-T-A-A-A-T-C-T 18 bases

DOI no: 10.1038/28101

Nature 394:145-151 (1998)

PubMed id: 9671298

Three-dimensional structure of the Stat3beta homodimer bound to DNA.

S.Becker, B.Groner, C.W.Müller.

ABSTRACT

STAT proteins are a family of eukaryotic transcription factors that mediate the response to a large number of cytokines and growth factors. Upon activation by cell-surface receptors or their associated kinases, STAT proteins dimerize, translocate to the nucleus and bind to specific promoter sequences on their target genes. Here we report the first crystal structure of a STAT protein bound to its DNA recognition site at 2.25 A resolution. The structure provides insight into the various steps by which STAT proteins deliver a response signal directly from the cell membrane to their target genes in the nucleus.

Selected figure(s)

Figure 2.
Figure 2 Ribbon diagram of the Stat3 homodimer-DNA complex. The N-terminal 4-helix bundle is shown in blue, the -barrel domain in red, the connector domain in green, and the SH2 domain and phosphotyrosine-containing region in yellow. Disordered regions between helices 1 and 2 and residues 689 to 701 have been modelled in grey. This figure and Fig. 3b were produced with program RIBBONS45. Views are shown a, along the DNA axis (the dyad of the complex running vertically); b, from the side, with monomer 2 depicted in grey; and c, from the top. Phosphotyrosines are indicated by a Y in c.

Figure 4.
Figure 4 Binding of the phosphotyrosine peptides by the C-terminal SH2 domains. Carbon atoms of the phosphotyrosine peptide covalently linked to monomer I and monomer II are yellow and white, respectively. Produced with program GRASP46.

The above figures are reprinted by permission from Macmillan Publishers Ltd: Nature (1998, 394, 145-151) copyright 1998.

Figures were selected by the author.