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PDBsum entry 1aj5
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Calcium-binding protein
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PDB id
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1aj5
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of a calpain ca(2+)-Binding domain reveals a novel ef-Hand and ca(2+)-Induced conformational changes.
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Authors
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H.Blanchard,
P.Grochulski,
Y.Li,
J.S.Arthur,
P.L.Davies,
J.S.Elce,
M.Cygler.
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Ref.
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Nat Struct Biol, 1997,
4,
532-538.
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PubMed id
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Abstract
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The crystal structure of a Ca(2+)-binding domain (dVI) of rat m-calpain has been
determined at 2.3 A resolution, both with and without bound Ca2+. The structures
reveal a unique fold incorporating five EF-hand motifs per monomer, three of
which bind calcium at physiological calcium concentrations, with one showing a
novel EF-hand coordination pattern. This investigation gives us a first view of
the calcium-induced conformational changes, and consequently an insight into the
mechanism of calcium induced activation in calpain. The crystal structures
reveal a dVI homodimer which provides a preliminary model for the subunit
dimerization in calpain.
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Secondary reference #1
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Title
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Ca(2+)-Binding domain VI of rat calpain is a homodimer in solution: hydrodynamic, Crystallization and preliminary X-Ray diffraction studies.
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Authors
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H.Blanchard,
Y.Li,
M.Cygler,
C.M.Kay,
J.Simon,
C.Arthur,
P.L.Davies,
J.S.Elce.
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Ref.
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Protein Sci, 1996,
5,
535-537.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Active recombinant rat calpain ii. Bacterially produced large and small subunits associate both in vivo and in vitro.
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Authors
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K.Graham-Siegenthaler,
S.Gauthier,
P.L.Davies,
J.S.Elce.
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Ref.
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J Biol Chem, 1994,
269,
30457-30460.
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PubMed id
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