PDBsum entry 1aey

Cytoskeleton

PDB id: 1aey

Contents

Protein chain

58 a.a.

References listed in PDB file

Key reference

• Title: 1h and 15n nmr assignment and solution structure of the sh3 domain of spectrin: comparison of unrefined and refined structure sets with the crystal structure.
• Authors: F.J.Blanco, A.R.Ortiz, L.Serrano.
• Ref.: J Biomol Nmr, 1997, 9, 347-357.
• PubMed id: 9255941

Abstract

The assignment of the 1H and 15N nuclear magnetic resonance spectra of the Src-homology region 3 domain of chicken brain alpha-spectrin has been obtained. A set of solution structures has been determined from distance and dihedral angle restraints, which provide a reasonable representation of the protein structure in solution, as evaluated by a principal component analysis of the global pairwise root-mean-square deviation (rmsd) in a large set of structures consisting of the refined and unrefined solution structures and the crystal structure. The solution structure is well defined, with a lower degree of convergence between the structures in the loop regions than in the secondary structure elements. The average pairwise rmsd between the 15 refined solution structures is 0.71 +/- 0.13 A for the backbone atoms and 1.43 +/- 0.14 A for all heavy atoms. The solution structure is basically the same as the crystal structure. The average rmsd between the 15 refined solution structures and the crystal structure is 0.76 A for the backbone atoms and 1.45 +/- 0.09 A for all heavy atoms. There are, however, small differences probably caused by intermolecular contacts in the crystal structure.

Secondary reference #1

• Title: Different folding transition states may result in the same native structure.
• Authors: A.R.Viguera, L.Serrano, M.Wilmanns.
• Ref.: Nat Struct Biol, 1996, 3, 874-880.
• PubMed id: 8836105

Secondary reference #2

• Title: Crystal structure of a src-Homology 3 (sh3) domain.
• Authors: A.Musacchio, M.Noble, R.Pauptit, R.Wierenga, M.Saraste.
• Ref.: Nature, 1992, 359, 851-855.
• PubMed id: 1279434