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PDBsum entry 1adn
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Transcription regulation
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PDB id
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1adn
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References listed in PDB file
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Key reference
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Title
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Solution structure of the DNA methyl phosphotriester repair domain of escherichia coli ada.
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Authors
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L.C.Myers,
G.L.Verdine,
G.Wagner.
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Ref.
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Biochemistry, 1993,
32,
14089-14094.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
88%.
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Abstract
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The Escherichia coli Ada protein repairs methyl phosphotriesters in DNA by
direct, irreversible methyl transfer to one of its own cysteine residues. The
methyl-transfer process appears to be autocatalyzed by coordination of the
acceptor residue, Cys-69, to a tightly bound zinc ion. Upon methyl transfer, Ada
acquires the ability to bind DNA sequence-specifically and thereby to induce
genes that confer resistance to methylating agents. The solution structure of an
N-terminal 10-kDa fragment of Ada, which retains zinc binding and DNA methyl
phosphotriester repair activities, was determined using multidimensional
heteronuclear nuclear magnetic resonance techniques. The structure reveals a
zinc-binding motif unlike any observed thus far in transcription factors or
zinc-containing enzymes and provides insight into the mechanism of
metalloactivated DNA repair.
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Secondary reference #1
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Title
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Repair of DNA methylphosphotriesters through a metalloactivated cysteine nucleophile.
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Authors
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L.C.Myers,
M.P.Terranova,
A.E.Ferentz,
G.Wagner,
G.L.Verdine.
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Ref.
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Science, 1993,
261,
1164-1167.
[DOI no: ]
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PubMed id
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