PDBsum entry 1acf

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protein links
Contractile protein PDB id
Protein chain
125 a.a. *
Waters ×64
* Residue conservation analysis
PDB id:
Name: Contractile protein
Title: Acanthamoeba castellanii profilin ib
Structure: Profilin i. Chain: a. Engineered: yes
Source: Acanthamoeba castellanii. Organism_taxid: 5755
2.00Å     R-factor:   0.179    
Authors: A.A.Fedorov,K.A.Magnus,M.H.Graupe,E.E.Lattman,T.D.Pollard, S.C.Almo
Key ref: A.A.Fedorov et al. (1994). X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates. Proc Natl Acad Sci U S A, 91, 8636-8640. PubMed id: 8078936 DOI: 10.1073/pnas.91.18.8636
29-Jul-94     Release date:   31-Aug-94    
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Protein chain
Pfam   ArchSchema ?
Q95VF7  (PRO1B_ACACA) -  Profilin-1B
126 a.a.
125 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   2 terms 
  Biological process     actin cytoskeleton organization   1 term 
  Biochemical function     actin binding     1 term  


DOI no: 10.1073/pnas.91.18.8636 Proc Natl Acad Sci U S A 91:8636-8640 (1994)
PubMed id: 8078936  
X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates.
A.A.Fedorov, K.A.Magnus, M.H.Graupe, E.E.Lattman, T.D.Pollard, S.C.Almo.
We determined the structures of Acanthamoeba profilin I and profilin II by x-ray crystallography at resolutions of 2.0 and 2.8 A, respectively. The polypeptide folds and the actin-binding surfaces of the amoeba profilins are very similar to those of bovine and human profilins. The electrostatic potential surfaces of the two Acanthamoeba isoforms differ. Two areas of high positive potential on the surface of profilin II are candidate binding sites for phosphatidylinositol phosphates. The proximity of these sites to the actin binding site provides an explanation for the competition between actin and lipids for binding profilin.

Literature references that cite this PDB file's key reference

  PubMed id Reference
19419568 M.Behnen, K.Murk, P.Kursula, H.Cappallo-Obermann, M.Rothkegel, A.L.Kierszenbaum, and C.Kirchhoff (2009).
Testis-expressed profilins 3 and 4 show distinct functional characteristics and localize in the acroplaxome-manchette complex in spermatids.
  BMC Cell Biol, 10, 34.  
19289059 R.Kardos, K.Pozsonyi, E.Nevalainen, P.Lappalainen, M.Nyitrai, and G.Hild (2009).
The effects of ADF/cofilin and profilin on the conformation of the ATP-binding cleft of monomeric actin.
  Biophys J, 96, 2335-2343.  
19307711 T.Haikarainen, W.Q.Chen, G.Lubec, and P.Kursula (2009).
Structure, modifications and ligand-binding properties of rat profilin 2a.
  Acta Crystallogr D Biol Crystallogr, 65, 303-311.
PDB code: 2vk3
18778430 S.Morales, J.C.Jiménez-López, A.J.Castro, M.I.Rodríguez-García, and J.D.Alché (2008).
Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination.
  J Microsc, 231, 332-341.  
17193263 A.J.Bojarski, M.Nowak, and B.Testa (2006).
Conformational fluctuations versus constraints in amino acid side chains: the evolution of information content from free amino acids to proteins.
  Chem Biodivers, 3, 245-273.  
16317718 D.Polet, A.Lambrechts, K.Ono, A.Mah, F.Peelman, J.Vandekerckhove, D.L.Baillie, C.Ampe, and S.Ono (2006).
Caenorhabditis elegans expresses three functional profilins in a tissue-specific manner.
  Cell Motil Cytoskeleton, 63, 14-28.  
15880548 L.J.Ball, R.Kühne, J.Schneider-Mergener, and H.Oschkinat (2005).
Recognition of Proline-Rich Motifs by Protein-Protein-Interaction Domains.
  Angew Chem Int Ed Engl, 44, 2852-2869.  
15308213 W.Witke (2004).
The role of profilin complexes in cell motility and other cellular processes.
  Trends Cell Biol, 14, 461-469.  
12052260 A.Lambrechts, V.Jonckheere, D.Dewitte, J.Vandekerckhove, and C.Ampe (2002).
Mutational analysis of human profilin I reveals a second PI(4,5)-P2 binding site neighbouring the poly(L-proline) binding site.
  BMC Biochem, 3, 12.  
  11294914 J.Lu, and T.D.Pollard (2001).
Profilin binding to poly-L-proline and actin monomers along with ability to catalyze actin nucleotide exchange is required for viability of fission yeast.
  Mol Biol Cell, 12, 1161-1175.  
11015196 M.Van Troys, D.Dewitte, J.L.Verschelde, M.Goethals, J.Vandekerckhove, and C.Ampe (2000).
The competitive interaction of actin and PIP2 with actophorin is based on overlapping target sites: design of a gain-of-function mutant.
  Biochemistry, 39, 12181-12189.  
9990286 M.Van Troys, J.Vandekerckhove, and C.Ampe (1999).
Structural modules in actin-binding proteins: towards a new classification.
  Biochim Biophys Acta, 1448, 323-348.  
9687532 D.D.Billadeau, K.M.Brumbaugh, C.J.Dick, R.A.Schoon, X.R.Bustelo, and P.J.Leibson (1998).
The Vav-Rac1 pathway in cytotoxic lymphocytes regulates the generation of cell-mediated killing.
  J Exp Med, 188, 549-559.  
9698363 J.C.Eads, N.M.Mahoney, S.Vorobiev, A.R.Bresnick, K.K.Wen, P.A.Rubenstein, B.K.Haarer, and S.C.Almo (1998).
Structure determination and characterization of Saccharomyces cerevisiae profilin.
  Biochemistry, 37, 11171-11181.
PDB code: 1ypr
9692980 V.K.Vinson, E.M.De La Cruz, H.N.Higgs, and T.D.Pollard (1998).
Interactions of Acanthamoeba profilin with actin and nucleotides bound to actin.
  Biochemistry, 37, 10871-10880.  
9484592 Y.A.Puius, N.M.Mahoney, and S.C.Almo (1998).
The modular structure of actin-regulatory proteins.
  Curr Opin Cell Biol, 10, 23-34.  
9145106 A.A.Fedorov, P.Lappalainen, E.V.Fedorov, D.G.Drubin, and S.C.Almo (1997).
Structure determination of yeast cofilin.
  Nat Struct Biol, 4, 366-369.
PDB codes: 1cfy 1cof 1qpv
9016715 A.A.Fedorov, T.Ball, N.M.Mahoney, R.Valenta, and S.C.Almo (1997).
The molecular basis for allergen cross-reactivity: crystal structure and IgE-epitope mapping of birch pollen profilin.
  Structure, 5, 33-45.
PDB code: 1cqa
9224949 J.A.Asturias, M.C.Arilla, B.Bartolomé, J.Martínez, A.Martínez, and R.Palacios (1997).
Sequence polymorphism and structural analysis of timothy grass pollen profilin allergen (Phl p 11).
  Biochim Biophys Acta, 1352, 253-257.  
9164454 K.Djinovic Carugo, S.Bañuelos, and M.Saraste (1997).
Crystal structure of a calponin homology domain.
  Nat Struct Biol, 4, 175-179.
PDB code: 1aa2
9016723 K.S.Thorn, H.E.Christensen, R.Shigeta, D.Huddler, L.Shalaby, U.Lindberg, N.H.Chua, and C.E.Schutt (1997).
The crystal structure of a major allergen from plants.
  Structure, 5, 19-32.
PDB codes: 1a0k 1plm 3nul
9409807 K.Schlüter, B.M.Jockusch, and M.Rothkegel (1997).
Profilins as regulators of actin dynamics.
  Biochim Biophys Acta, 1359, 97.  
9284311 L.A.Flanagan, C.C.Cunningham, J.Chen, G.D.Prestwich, K.S.Kosik, and P.A.Janmey (1997).
The structure of divalent cation-induced aggregates of PIP2 and their alteration by gelsolin and tau.
  Biophys J, 73, 1440-1447.  
9096955 L.Vidali, and P.K.Hepler (1997).
Characterization and localization of profilin in pollen grains and tubes of Lilium longiflorum.
  Cell Motil Cytoskeleton, 36, 323-338.  
8987987 E.C.Petrella, L.M.Machesky, D.A.Kaiser, and T.D.Pollard (1996).
Structural requirements and thermodynamics of the interaction of proline peptides with profilin.
  Biochemistry, 35, 16535-16543.  
8860230 I.Karakesisoglou, M.Schleicher, B.C.Gibbon, and C.J.Staiger (1996).
Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells.
  Cell Motil Cytoskeleton, 34, 36-47.  
  8978670 M.K.Balasubramanian, A.Feoktistova, D.McCollum, and K.L.Gould (1996).
Fission yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin function.
  EMBO J, 15, 6426-6437.  
8909300 P.J.Lu, W.R.Shieh, S.G.Rhee, H.L.Yin, and C.S.Chen (1996).
Lipid products of phosphoinositide 3-kinase bind human profilin with high affinity.
  Biochemistry, 35, 14027-14034.  
7601111 A.Lambrechts, J.van Damme, M.Goethals, J.Vandekerckhove, and C.Ampe (1995).
Purification and characterization of bovine profilin II. Actin, poly(L-proline) and inositolphospholipid binding.
  Eur J Biochem, 230, 281-286.  
7758455 R.Gieselmann, D.J.Kwiatkowski, P.A.Janmey, and W.Witke (1995).
Distinct biochemical characteristics of the two human profilin isoforms.
  Eur J Biochem, 229, 621-628.  
  7795529 W.J.Metzler, B.T.Farmer, K.L.Constantine, M.S.Friedrichs, T.Lavoie, and L.Mueller (1995).
Refined solution structure of human profilin I.
  Protein Sci, 4, 450-459.
PDB code: 1pfl
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.