PDBsum entry 1aa7

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Matrix protein PDB id
Jmol PyMol
Protein chains
158 a.a.
Waters ×258
PDB id:
Name: Matrix protein
Title: Influenza virus matrix protein crystal structure at ph 4.0
Structure: Influenza virus matrix protein. Chain: a, b
Source: Unidentified influenza virus. Organism_taxid: 11309. Strain: a-pr8
Biol. unit: Dimer (from PDB file)
2.08Å     R-factor:   0.208     R-free:   0.280
Authors: B.Sha,M.Luo
Key ref: B.Sha and M.Luo (1997). Structure of a bifunctional membrane-RNA binding protein, influenza virus matrix protein M1. Nat Struct Biol, 4, 239-244. PubMed id: 9164466
24-Jan-97     Release date:   28-Jan-98    
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Protein chains
Pfam   ArchSchema ?
P03485  (M1_I34A1) -  Matrix protein 1
252 a.a.
158 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     structural molecule activity     2 terms  


Nat Struct Biol 4:239-244 (1997)
PubMed id: 9164466  
Structure of a bifunctional membrane-RNA binding protein, influenza virus matrix protein M1.
B.Sha, M.Luo.
Matrix protein (M1) of influenza virus is a bifunctional protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. It is therefore required that M1 binds both membrane and RNA simultaneously. The X-ray crystal structure of the N-terminal portion of type A influenza virus M1-amino acid residues 2-158-has been determined at 2.08 A resolution at pH 4.0. The protein forms a dimer. A highly positively charged region on the dimer surface is suitably positioned to bind RNA while the hydrophobic surface opposite the RNA binding region may be involved in interactions with the membrane. The membrane-binding hydrophobic surface could be buried or exposed after a conformational change.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21166891 K.F.Block, E.Puerta-Fernandez, J.G.Wallace, and R.R.Breaker (2011).
Association of OLE RNA with bacterial membranes via an RNA-protein interaction.
  Mol Microbiol, 79, 21-34.  
19898475 J.Wang, R.M.Pielak, M.A.McClintock, and J.J.Chou (2009).
Solution structure and functional analysis of the influenza B proton channel.
  Nat Struct Mol Biol, 16, 1267-1271.
PDB codes: 2kix 2kj1
19656971 J.Zhang, G.Li, X.Liu, Z.Wang, W.Liu, and X.Ye (2009).
Influenza A virus M1 blocks the classical complement pathway through interacting with C1qA.
  J Gen Virol, 90, 2751-2758.  
19237566 P.Neumann, D.Lieber, S.Meyer, P.Dautel, A.Kerth, I.Kraus, W.Garten, and M.T.Stubbs (2009).
Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals ssRNA binding properties.
  Proc Natl Acad Sci U S A, 106, 3710-3715.
PDB code: 3f1j
19521658 S.Huang, J.Chen, H.Wang, B.Sun, H.Wang, Z.Zhang, X.Zhang, and Z.Chen (2009).
Influenza A virus matrix protein 1 interacts with hTFIIIC102-s, a short isoform of the polypeptide 3 subunit of human general transcription factor IIIC.
  Arch Virol, 154, 1101-1110.  
18768984 C.Chiang, G.W.Chen, and S.R.Shih (2008).
Mutations at alternative 5' splice sites of M1 mRNA negatively affect influenza A virus viability and growth rate.
  J Virol, 82, 10873-10886.  
18672693 I.u.A.Smirnova, L.V.Kordiukova, M.V.Serebriakova, I.I.u.Filippova, E.N.Lysogorskaia, and L.A.Baratova (2008).
[Flu virion as a substrate for proteolytic enzymes]
  Bioorg Khim, 34, 409-415.  
18400345 P.O.Ilyinskii, A.B.Meriin, V.L.Gabai, O.P.Zhirnov, G.Thoidis, and A.M.Shneider (2008).
Prime-boost vaccination with a combination of proteosome-degradable and wild-type forms of two influenza proteins leads to augmented CTL response.
  Vaccine, 26, 2177-2185.  
18725937 T.T.Lam, C.C.Hon, O.G.Pybus, S.L.Kosakovsky Pond, R.T.Wong, C.W.Yip, F.Zeng, and F.C.Leung (2008).
Evolutionary and transmission dynamics of reassortant H5N1 influenza virus in Indonesia.
  PLoS Pathog, 4, e1000130.  
17622633 S.L.Noton, E.Medcalf, D.Fisher, A.E.Mullin, D.Elton, and P.Digard (2007).
Identification of the domains of the influenza A virus M1 matrix protein required for NP binding, oligomerization and incorporation into virions.
  J Gen Virol, 88, 2280-2290.  
16474136 E.K.Hui, S.Barman, D.H.Tang, B.France, and D.P.Nayak (2006).
YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42.
  J Virol, 80, 2291-2308.  
16227287 B.J.Chen, M.Takeda, and R.A.Lamb (2005).
Influenza virus hemagglutinin (H3 subtype) requires palmitoylation of its cytoplasmic tail for assembly: M1 proteins of two subtypes differ in their ability to support assembly.
  J Virol, 79, 13673-13684.  
15661164 I.Garcia-Robles, H.Akarsu, C.W.Müller, R.W.Ruigrok, and F.Baudin (2005).
Interaction of influenza virus proteins with nucleosomes.
  Virology, 332, 329-336.  
15892972 J.A.McCullers, E.Hoffmann, V.C.Huber, and A.D.Nickerson (2005).
A single amino acid change in the C-terminal domain of the matrix protein M1 of influenza B virus confers mouse adaptation and virulence.
  Virology, 336, 318-326.  
15613353 L.M.Burleigh, L.J.Calder, J.J.Skehel, and D.A.Steinhauer (2005).
Influenza a viruses with mutations in the m1 helix six domain display a wide variety of morphological phenotypes.
  J Virol, 79, 1262-1270.  
15108720 J.Timmins, R.W.Ruigrok, and W.Weissenhorn (2004).
Structural studies on the Ebola virus matrix protein VP40 indicate that matrix proteins of enveloped RNA viruses are analogues but not homologues.
  FEMS Microbiol Lett, 233, 179-186.  
15340924 K.B.Murray, W.R.Taylor, and J.M.Thornton (2004).
Toward the detection and validation of repeats in protein structure.
  Proteins, 57, 365-380.  
14993605 L.Ma, C.T.Jones, T.D.Groesch, R.J.Kuhn, and C.B.Post (2004).
Solution structure of dengue virus capsid protein reveals another fold.
  Proc Natl Acad Sci U S A, 101, 3414-3419.
PDB code: 1r6r
15331690 T.Liu, and Z.Ye (2004).
Introduction of a temperature-sensitive phenotype into influenza A/WSN/33 virus by altering the basic amino acid domain of influenza virus matrix protein.
  J Virol, 78, 9585-9591.  
12768027 E.K.Hui, S.Barman, T.Y.Yang, and D.P.Nayak (2003).
Basic residues of the helix six domain of influenza virus M1 involved in nuclear translocation of M1 can be replaced by PTAP and YPDL late assembly domain motifs.
  J Virol, 77, 7078-7092.  
12970177 H.Akarsu, W.P.Burmeister, C.Petosa, I.Petit, C.W.Müller, R.W.Ruigrok, and F.Baudin (2003).
Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2).
  EMBO J, 22, 4646-4655.
PDB code: 1pd3
12368314 A.H.Reid, T.G.Fanning, T.A.Janczewski, S.McCall, and J.K.Taubenberger (2002).
Characterization of the 1918 "Spanish" influenza virus matrix gene segment.
  J Virol, 76, 10717-10723.  
12065402 M.Gaudier, Y.Gaudin, and M.Knossow (2002).
Crystal structure of vesicular stomatitis virus matrix protein.
  EMBO J, 21, 2886-2892.
PDB code: 1lg7
12438632 T.Liu, and Z.Ye (2002).
Restriction of viral replication by mutation of the influenza virus matrix protein.
  J Virol, 76, 13055-13061.  
11226219 J.E.Padilla, C.Colovos, and T.O.Yeates (2001).
Nanohedra: using symmetry to design self assembling protein cages, layers, crystals, and filaments.
  Proc Natl Acad Sci U S A, 98, 2217-2221.  
11453070 S.Tchatalbachev, R.Flick, and G.Hobom (2001).
The packaging signal of influenza viral RNA molecules.
  RNA, 7, 979-989.  
10954572 A.Ali, R.T.Avalos, E.Ponimaskin, and D.P.Nayak (2000).
Influenza virus assembly: effect of influenza virus glycoproteins on the membrane association of M1 protein.
  J Virol, 74, 8709-8719.  
10944105 A.Dessen, V.Volchkov, O.Dolnik, H.D.Klenk, and W.Weissenhorn (2000).
Crystal structure of the matrix protein VP40 from Ebola virus.
  EMBO J, 19, 4228-4236.
PDB code: 1es6
11090151 P.Gómez-Puertas, C.Albo, E.Pérez-Pastrana, A.Vivo, and A.Portela (2000).
Influenza virus matrix protein is the major driving force in virus budding.
  J Virol, 74, 11538-11547.  
10393906 A.V.Shishkov, V.I.Goldanskii, L.A.Baratova, N.V.Fedorova, A.L.Ksenofontov, O.P.Zhirnov, and A.V.Galkin (1999).
The in situ spatial arrangement of the influenza A virus matrix protein M1 assessed by tritium bombardment.
  Proc Natl Acad Sci U S A, 96, 7827-7830.  
  10438836 Z.Ye, T.Liu, D.P.Offringa, J.McInnis, and R.A.Levandowski (1999).
Association of influenza virus matrix protein with ribonucleoproteins.
  J Virol, 73, 7467-7473.  
15991949 C.Cianci, and M.Krystal (1998).
Development of antivirals against influenza.
  Expert Opin Investig Drugs, 7, 149-165.  
  9841669 H.Garoff, R.Hewson, and D.J.Opstelten (1998).
Virus maturation by budding.
  Microbiol Mol Biol Rev, 62, 1171-1190.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.