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PDBsum entry 1a7d
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Oxygen transport
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PDB id
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1a7d
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References listed in PDB file
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Key reference
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Title
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Structures of wild-Type chloromet and l103n hydroxomet themiste zostericola myohemerythrins at 1.8 a resolution.
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Authors
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L.J.Martins,
C.P.Hill,
W.R.Ellis.
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Ref.
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Biochemistry, 1997,
36,
7044-7049.
[DOI no: ]
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PubMed id
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Abstract
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Myohemerythrin (Mhr) is a nonheme iron oxygen carrier found in the retractor
muscles of marine "peanut" worms. The X-ray crystal structures of two
recombinant Themiste zostericola Mhrs are reported to a resolution of 1.8 A.
Surprisingly, the met wild-type structure (R = 17.8%) was found to contain
chloride bound to Fe2, while coordinated hydroxide was found in the met L103N
structure (R = 18.3%). An internal water molecule was also found distal to the
Fe-O-Fe center of the mutant protein, forming hydrogen bonds with the
coordinated hydroxide and the OD1 atom of Asn-103. This finding is consistent
with the kinetic and spectroscopic results reported for the L103N mutant Mhr
[Raner, G. M., Martins, L. J., & Ellis, W. R., Jr. (1997) Biochemistry 36,
7037-7043]. Possible roles for the side chain of residue 103 (Leu in wild-type
Mhr) in gating ligand binding are also discussed.
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