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PDBsum entry 1a5t
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the delta' Subunit of the clamp-Loader complex of e. Coli DNA polymerase III.
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Authors
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B.Guenther,
R.Onrust,
A.Sali,
M.O'Donnell,
J.Kuriyan.
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Ref.
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Cell, 1997,
91,
335-345.
[DOI no: ]
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PubMed id
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Abstract
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The crystal structure of the delta' subunit of the clamp-loader complex of E.
coli DNA polymerase III has been determined. Three consecutive domains in the
structure are arranged in a C-shaped architecture. The N-terminal domain
contains a nonfunctional nucleotide binding site. The catalytic component of the
clamp-loader complex is the gamma subunit, which is homologous to delta'. A
sequence-structure alignment suggests that nucleotides bind to gamma at an
interdomain interface within the inner surface of the "C." The
alignment is extended to other clamp-loader complexes and to the RuvB family of
DNA helicases, and suggests that each of these is assembled from C-shaped
components that can open and close the jaws of the "C" in response to
ATP binding and hydrolysis.
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Figure 5.
Figure 5. Inactive Nucleotide-Binding Region of δ′The
region surrounding the P loop of δ′ is shown, with side
chains of certain residues indicated in stick representation.
P-loop sequences and sensor sequences in δ′ and other
proteins are indicated. The structure of ATP is taken from
adenylate kinase, after the P-loop segments of adenylate kinase
and δ′ were superimposed. Residues that are actual or
potential ligands of the phosphate groups of ATP are highlighed
in blue in the alignment of sequence motifs.
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Figure 6.
Figure 6. GRASP Diagrams of δ′The molecular surface of
δ′ is shown in two orientations, colored according to local
electrostatic potential. A transparent representation of the
surface with the polypeptide backbone revealed underneath is
also shown. Note that there are no contacts between Domains 1
and 3, and that the appearance of such contacts in the GRASP
diagram is a consequence of the orientation of the molecule.
Figure prepared using GRASP ([32]).
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The above figures are
reprinted
by permission from Cell Press:
Cell
(1997,
91,
335-345)
copyright 1997.
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Secondary reference #1
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Title
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Author
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B.D.Guenther.
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Ref.
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structural studies on the ...
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