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PDBsum entry 1a5j
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DNA binding protein
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PDB id
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1a5j
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References listed in PDB file
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Key reference
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Title
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Solution structure of the b-Myb DNA-Binding domain: a possible role for conformational instability of the protein in DNA binding and control of gene expression.
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Authors
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P.B.Mcintosh,
T.A.Frenkiel,
U.Wollborn,
J.E.Mccormick,
K.H.Klempnauer,
J.Feeney,
M.D.Carr.
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Ref.
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Biochemistry, 1998,
37,
9619-9629.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
88%.
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Abstract
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Double- and triple-resonance heteronuclear NMR spectroscopy have been used to
determine the high-resolution solution structure of the minimal B-Myb
DNA-binding domain (B-MybR2R3) and to characterize the specific complex formed
with a synthetic DNA fragment corresponding to the Myb target site on the
Myb-regulated gene tom-1. B-MybR2R3 is shown to consist of two independent
protein domains (R2 and R3) joined by a short linker, which have strikingly
different tertiary structures despite significant sequence similarities. In
addition, the C-terminal region of B-Myb R2 is confirmed to have a poorly
defined structure, reflecting the existence of multiple conformations in slow to
intermediate exchange. This contrasts with the tertiary structure reported for
c-MybR2R3, in which both R2 and R3 have the same fold and the C-terminal region
of R2 forms a stable, well-defined helix [Ogata, K., et al. (1995) Nat. Struct.
Biol. 2, 309-320]. The NMR data suggest there are extensive contacts between
B-MybR2R3 and its DNA target site in the complex and are consistent with a
significant conformational change in the protein on binding to DNA, with one
possibility being the formation of a stable helix in the C-terminal region of
R2. In addition, conformational heterogeneity identified in R2 of B-MybR2R3
bound to the tom-1-A target site may play an important role in the control of
gene expression by Myb proteins.
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Secondary reference #1
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Title
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Structure of the b-Myb DNA-Binding domain in solution and evidence for multiple conformations in the region of repeat-2 involved in DNA binding: implications for sequence-Specific DNA binding by myb proteins.
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Authors
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M.D.Carr,
U.Wollborn,
P.B.Mcintosh,
T.A.Frenkiel,
J.E.Mccormick,
C.J.Bauer,
K.H.Klempnauer,
J.Feeney.
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Ref.
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Eur J Biochem, 1996,
235,
721-735.
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PubMed id
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