|
|
||
|
|
|
|
|
UniProt functional annotation for P00550 | ||
|
|
|
|
|
|
||
| UniProt code: P00550. |
|
||
| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
|
||
|
||
| Function: | |
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in D-mannitol transport (PubMed:368051, PubMed:6427236, PubMed:2123863). Also able to use D-mannonic acid (PubMed:6427236). {ECO:0000269|PubMed:2123863, ECO:0000269|PubMed:368051, ECO:0000269|PubMed:6427236}. |
|
||
| Catalytic activity: | |
Reaction=D-mannitol(out) + N(pros)-phospho-L-histidyl-[protein] = D- mannitol 1-phosphate(in) + L-histidyl-[protein]; Xref=Rhea:RHEA:33363, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:16899, ChEBI:CHEBI:29979, ChEBI:CHEBI:61381, ChEBI:CHEBI:64837; EC=2.7.1.197; Evidence={ECO:0000269|PubMed:2123863, ECO:0000305|PubMed:368051}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=66 uM for mannitol phosphorylation {ECO:0000269|PubMed:2123863}; Vmax=21.7 nmol/min/mg enzyme toward mannitol {ECO:0000269|PubMed:2123863}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:3322385}. |
| Subcellular location: | |
Cell inner membrane {ECO:0000255|PROSITE- ProRule:PRU00427, ECO:0000269|PubMed:15919996, ECO:0000305|PubMed:6309813}; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00427, ECO:0000269|PubMed:15919996, ECO:0000305|PubMed:368051}. |
| Induction: | |
Induced by mannitol. Repressed by MltR. {ECO:0000269|PubMed:8300537}. |
| Domain: | |
The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site. {ECO:0000255|PROSITE-ProRule:PRU00427}. |
| Domain: | |
The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain. {ECO:0000255|PROSITE-ProRule:PRU00422}. |
| Domain: | |
The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain. {ECO:0000255|PROSITE-ProRule:PRU00417}. |
| Ptm: | |
An intramolecular phosphotransfer takes places between His-554 and Cys-384. {ECO:0000305|PubMed:16443929}. |
Annotations taken from UniProtKB at the EBI.