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UniProt functional annotation for P0A9D2 | ||
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| UniProt code: P0A9D2. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the conjugation of reduced glutathione (GSH) to a wide number of exogenous and endogenous hydrophobic electrophiles. Shows activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and ethacrynic acid. Also possesses thiol:disulfide oxidoreductase activity, using GSH to reduce bis-(2-hydroxyethyl) disulfide (HEDS). Has a low level of glutathione-dependent peroxidase activity toward cumene hydroperoxide. Is important for defense against oxidative stress, probably via its peroxidase activity. {ECO:0000269|PubMed:17018556, ECO:0000269|PubMed:18778244, ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}. |
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| Catalytic activity: | |
Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269|PubMed:18778244, ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.041 mM for glutathione {ECO:0000269|PubMed:18778244, ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}; KM=0.3 mM for glutathione {ECO:0000269|PubMed:18778244, ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}; KM=0.99 mM for 1-chloro-2,4-dinitrobenzene {ECO:0000269|PubMed:18778244, ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}; KM=1.9 mM for 1-chloro-2,4-dinitrobenzene {ECO:0000269|PubMed:18778244, ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}; Note=kcat is 9 sec(-1) for the GSH transferase reaction with CDNB as substrate. {ECO:0000269|PubMed:18778244}; pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}; Temperature dependence: Optimum temperature is 35 degrees Celsius. {ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:14635120, ECO:0000269|PubMed:2185038, ECO:0000269|PubMed:7798255, ECO:0000269|PubMed:9680481}. |
| Subcellular location: | |
Cytoplasm. |
| Disruption phenotype: | |
Deletion of the gstA gene decreases the resistance of the bacteria to hydrogen peroxide. {ECO:0000269|PubMed:17018556}. |
| Similarity: | |
Belongs to the GST superfamily. Beta family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.