 |
PDBsum entry 1a0b
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Histidine kinase
|
PDB id
|
|
|
|
1a0b
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Insights into multistep phosphorelay from the crystal structure of the c-Terminal hpt domain of arcb.
|
|
|
Authors
|
|
M.Kato,
T.Mizuno,
T.Shimizu,
T.Hakoshima.
|
|
|
Ref.
|
|
Cell, 1997,
88,
717-723.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The histidine-containing phosphotransfer (HPt) domain is a novel protein module
with an active histidine residue that mediates phosphotransfer reactions in the
two-component signaling systems. A multistep phosphorelay involving the HPt
domain has been suggested for these signaling pathways. The crystal structure of
the HPt domain of the anaerobic sensor kinase ArcB has been determined at 2.06 A
resolution. The domain consists of six alpha helices containing a four-helix
bundle-folding. The pattern of sequence similarity of the HPt domains of ArcB
and components in other signaling systems can be interpreted in light of the
three-dimensional structure and supports the conclusion that the HPt domains
have a common structural motif both in prokaryotes and eukaryotes.
|
|
|
|
|
|
|
|
Figure 4.
Figure 4. Helix Structure and Sequence Alignment of HPt
DomainsSide-view of helix D of the HPt domain of ArcB using a
ball-and-stick model with the 2F[o]-F[c] electron density map.
The imidazole ring makes a hydrogen bond to a water molecule (W).
|
|
Figure 5.
Figure 5. Comparison of HPt and P1 DomainsStructural
comparison of the HPt domain of ArcB (right) and the P1 domain
of CheA (left). The main chain is drawn as a ribbon in red for
helices D and E of the HPt domain of ArcB and the corresponding
part of CheA. The side chain of active His-717 of the HPt domain
and the location of the corresponding histidine in the P1 domain
are shown in green.
|
|
|
|
|
|
The above figures are
reprinted
by permission from Cell Press:
Cell
(1997,
88,
717-723)
copyright 1997.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Crystallization and preliminary X-Ray analysis of a histidine kinase domain of the anaerobic sensor protein arcb from escherichia coli.
|
|
|
Authors
|
|
M.Kato,
K.Ishige,
T.Mizuno,
T.Shimizu,
T.Hakoshima.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 1996,
52,
1214-1215.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
Figure 1.
Fig. 1. Crystal of ArcB
c. The scale bar is 0.5 mm long.
|
|
|
|
|
|
The above figure is
reproduced from the cited reference
with permission from the IUCr
|
|
|
|
|
|
|
