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PDBsum entry 1zuo

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protein ligands Protein-protein interface(s) links
Ligase PDB id
1zuo
Jmol
Contents
Protein chains
162 a.a. *
140 a.a. *
Ligands
BME ×2
Waters ×224
* Residue conservation analysis
PDB id:
1zuo
Name: Ligase
Title: Structure of human ubiquitin-conjugating enzyme (ubci) invol embryo attachment and implantation
Structure: Hypothetical protein loc92912. Chain: a, b. Fragment: catalytic domain, residues 201-363. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: loc92912. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.80Å     R-factor:   0.201     R-free:   0.232
Authors: J.R.Walker,G.V.Avvakumov,H.Cui,E.M.Newman,F.Mackenzie,M.Sund C.Arrowsmith,A.Edwards,A.Bochkarev,S.Dhe-Paganon,Structural Consortium (Sgc)
Key ref: Y.Sheng et al. (2012). A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen. Mol Cell Proteomics, 11, 329-341. PubMed id: 22496338 DOI: 10.1074/mcp.O111.013706
Date:
31-May-05     Release date:   05-Jul-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q8WVN8  (UB2Q2_HUMAN) -  Ubiquitin-conjugating enzyme E2 Q2
Seq:
Struc:
375 a.a.
162 a.a.
Protein chain
Pfam   ArchSchema ?
Q8WVN8  (UB2Q2_HUMAN) -  Ubiquitin-conjugating enzyme E2 Q2
Seq:
Struc:
375 a.a.
140 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.6.3.2.19  - Ubiquitin--protein ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
ATP
+ ubiquitin
+ protein lysine
= AMP
+ diphosphate
+ protein N-ubiquityllysine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     acid-amino acid ligase activity     1 term  

 

 
    reference    
 
 
DOI no: 10.1074/mcp.O111.013706 Mol Cell Proteomics 11:329-341 (2012)
PubMed id: 22496338  
 
 
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Y.Sheng, J.H.Hong, R.Doherty, T.Srikumar, J.Shloush, G.V.Avvakumov, J.R.Walker, S.Xue, D.Neculai, J.W.Wan, S.K.Kim, C.H.Arrowsmith, B.Raught, S.Dhe-Paganon.
 
  ABSTRACT  
 
No abstract given.