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PDBsum entry 1uvo
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* Residue conservation analysis
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Enzyme class:
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E.C.3.4.21.36
- pancreatic elastase.
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Reaction:
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Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
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J Synchrotron Radiat
12:304-309
(2005)
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PubMed id:
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On the influence of the incident photon energy on the radiation damage in crystalline biological samples.
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M.S.Weiss,
S.Panjikar,
C.Mueller-Dieckmann,
P.A.Tucker.
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ABSTRACT
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Two series of complete and highly redundant data sets were collected at
wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine
pancreatic elastase (PPE). Radiation damage to the sample was evaluated
qualitatively by inspecting consecutive difference electron density maps during
the course of the experiment. The nature of the radiation damage was found to be
identical at both wavelengths and was localized primarily at the four disulfide
bridges of PPE, the cadmium site and the two methionine residues. For a
quantitative examination of the radiation damage, the decrease in the peak
height of the cadmium ion in various electron density maps was exploited. Again,
no significant difference in radiation damage between the two wavelengths was
observed. This can be rationalized by considering the wavelength dependencies of
the number of diffracted photons versus the number of absorbed photons and the
energy deposited in the crystal by the latter.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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C.Homer,
L.Cooper,
and
A.Gonzalez
(2011).
Energy dependence of site-specific radiation damage in protein crystals.
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J Synchrotron Radiat,
18,
338-345.
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E.De la Mora,
I.Carmichael,
and
E.F.Garman
(2011).
Effective scavenging at cryotemperatures: further increasing the dose tolerance of protein crystals.
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J Synchrotron Radiat,
18,
346-357.
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PDB codes:
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E.F.Garman,
and
M.Weik
(2011).
Macromolecular crystallography radiation damage research: what's new?
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J Synchrotron Radiat,
18,
313-317.
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E.F.Garman
(2010).
Radiation damage in macromolecular crystallography: what is it and why should we care?
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Acta Crystallogr D Biol Crystallogr,
66,
339-351.
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K.S.Paithankar,
and
E.F.Garman
(2010).
Know your dose: RADDOSE.
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Acta Crystallogr D Biol Crystallogr,
66,
381-388.
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A.R.Crofts,
J.T.Holland,
D.Victoria,
D.R.Kolling,
S.A.Dikanov,
R.Gilbreth,
S.Lhee,
R.Kuras,
and
M.G.Kuras
(2008).
The Q-cycle reviewed: How well does a monomeric mechanism of the bc(1) complex account for the function of a dimeric complex?
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Biochim Biophys Acta,
1777,
1001-1019.
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B.Kauffmann,
M.S.Weiss,
V.S.Lamzin,
and
A.Schmidt
(2006).
How to avoid premature decay of your macromolecular crystal: a quick soak for long life.
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Structure,
14,
1099-1105.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
