PDBsum entry 1usy

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Transferase PDB id
Protein chains
275 a.a. *
202 a.a. *
HIS ×8
PO4 ×6
Waters ×274
* Residue conservation analysis
PDB id:
Name: Transferase
Title: Atp phosphoribosyl transferase (hisg:hisz) complex from thermotoga maritima
Structure: Atp phosphoribosyltransferase regulatory subunit. Chain: a, b, d. Engineered: yes. Atp phosphoribosyltransferase regulatory subunit. Chain: c. Engineered: yes. Atp phosphoribosyltransferase. Chain: e, f, g. Engineered: yes.
Source: Thermotoga maritima. Organism_taxid: 243274. Strain: msb8. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Octamer (from PDB file)
2.52Å     R-factor:   0.207     R-free:   0.287
Authors: M.C.Vega,F.J.Fernandez,G.E.Murphy,P.Zou,A.Popov,M.Wilmanns
Key ref: M.C.Vega et al. (2005). Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like subunit. Mol Microbiol, 55, 675-686. PubMed id: 15660995
01-Dec-03     Release date:   15-Dec-04    
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Protein chains
Pfam   ArchSchema ?
Q9X0D3  (HISZ_THEMA) -  ATP phosphoribosyltransferase regulatory subunit
275 a.a.
275 a.a.
Protein chains
Pfam   ArchSchema ?
Q9X0D2  (HIS1_THEMA) -  ATP phosphoribosyltransferase
208 a.a.
202 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     cellular amino acid biosynthetic process   2 terms 
  Biochemical function     nucleotide binding     5 terms  


Mol Microbiol 55:675-686 (2005)
PubMed id: 15660995  
Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like subunit.
M.C.Vega, P.Zou, F.J.Fernandez, G.E.Murphy, R.Sterner, A.Popov, M.Wilmanns.
The molecular structure of the ATP phosphoribosyl transferase from the hyperthermophile Thermotoga maritima is composed of a 220 kDa hetero-octameric complex comprising four catalytic subunits (HisGS) and four regulatory subunits (HisZ). Steady-state kinetics indicate that only the complete octameric complex is active and non-competitively inhibited by the pathway product histidine. The rationale for these findings is provided by the crystal structure revealing a total of eight histidine binding sites that are located within each of the four HisGS-HisZ subunit interfaces formed by the ATP phosphoribosyl transferase complex. While the structure of the catalytic HisGS subunit is related to the catalytic domain of another family of (HisGL)2 ATP phosphoribosyl transferases that is functional in the absence of additional regulatory subunits, the structure of the regulatory HisZ subunit is distantly related to class II aminoacyl-tRNA synthetases. However, neither the mode of the oligomeric subunit arrangement nor the type of histidine binding pockets is found in these structural relatives. Common ancestry of the regulatory HisZ subunit and class II aminoacyl-tRNA synthetase may reflect the balanced need of regulated amounts of a cognate amino acid (histidine) in the translation apparatus, ultimately linking amino acid biosynthesis and protein biosynthesis in terms of function, structure and evolution.

Literature references that cite this PDB file's key reference

  PubMed id Reference
19762480 F.Meyer, R.Overbeek, and A.Rodriguez (2009).
FIGfams: yet another set of protein families.
  Nucleic Acids Res, 37, 6643-6654.  
18778048 Y.Cho, T.R.Ioerger, and J.C.Sacchettini (2008).
Discovery of novel nitrobenzothiazole inhibitors for Mycobacterium tuberculosis ATP phosphoribosyl transferase (HisG) through virtual screening.
  J Med Chem, 51, 5984-5992.  
17154531 K.S.Champagne, E.Piscitelli, and C.S.Francklyn (2006).
Substrate recognition by the hetero-octameric ATP phosphoribosyltransferase from Lactococcus lactis.
  Biochemistry, 45, 14933-14943.  
16955235 R.Fani, M.Brilli, and P.Liò (2006).
Inference from proteobacterial operons shows piecewise organization: a reply to Price et al.
  J Mol Evol, 63, 577-580.  
16051603 K.S.Champagne, M.Sissler, Y.Larrabee, S.Doublié, and C.S.Francklyn (2005).
Activation of the hetero-octameric ATP phosphoribosyl transferase through subunit interface rearrangement by a tRNA synthetase paralog.
  J Biol Chem, 280, 34096-34104.
PDB codes: 1z7m 1z7n
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