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PDBsum entry 1ufv

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protein ligands metals Protein-protein interface(s) links
Ligase PDB id
1ufv
Jmol
Contents
Protein chains
276 a.a. *
Ligands
GOL ×5
Metals
_CL ×3
Waters ×478
* Residue conservation analysis
PDB id:
1ufv
Name: Ligase
Title: Crystal structure of pantothenate synthetase from thermus th hb8
Structure: Pantoate-beta-alanine ligase. Chain: a, b. Synonym: pantothenate synthetase. Engineered: yes. Mutation: yes. Other_details: mutation of val 48 to ala
Source: Thermus thermophilus. Organism_taxid: 274. Gene: panc. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Biol. unit: Dimer (from PQS)
Resolution:
2.05Å     R-factor:   0.201     R-free:   0.246
Authors: B.Bagautdinov,S.Kuramitsu,S.Yokoyama,M.Miyano,T.H.Tahirov,Ri Structural Genomics/proteomics Initiative (Rsgi)
Key ref: B.Bagautdinov et al. Crystal structure of pantothenate synthetase from thermus thermophilus hb8. To be published, .
Date:
10-Jun-03     Release date:   24-Jun-03    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q5SHF5  (PANC_THET8) -  Pantothenate synthetase
Seq:
Struc:
276 a.a.
276 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.6.3.2.1  - Pantoate--beta-alanine ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Coenzyme A Biosynthesis (early stages)
      Reaction: ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
ATP
+ (R)-pantoate
+
beta-alanine
Bound ligand (Het Group name = GOL)
matches with 50.00% similarity
= AMP
+ diphosphate
+ (R)-pantothenate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     biosynthetic process   2 terms 
  Biochemical function     catalytic activity     5 terms