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PDBsum entry 1t90

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
1t90
Jmol
Contents
Protein chains
484 a.a. *
Ligands
NAD ×4
Waters ×866
* Residue conservation analysis
PDB id:
1t90
Name: Oxidoreductase
Title: Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
Structure: Probable methylmalonate-semialdehyde dehydrogenase. Chain: a, b, c, d. Synonym: mmsdh. Engineered: yes
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: mmsa, iola, e83a, bsu39760. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Tetramer (from PQS)
Resolution:
2.50Å     R-factor:   0.211     R-free:   0.250
Authors: H.Dubourg,C.Didierjean,C.Stines-Chaumeil,F.Talfournier, G.Branlant,A.Aubry,C.Corbier
Key ref: H.Dubourg et al. Crystal structure analysis of methylmalonate-Semialdehyde dehydrogenase from bacillus subtilis.. To be published, .
Date:
14-May-04     Release date:   03-Jan-06    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P42412  (IOLA_BACSU) -  Methylmalonate semialdehyde dehydrogenase [acylating]
Seq:
Struc:
487 a.a.
484 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 1: E.C.1.2.1.18  - Malonate-semialdehyde dehydrogenase (acetylating).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 3-oxopropanoate + CoA + NAD(P)(+) = acetyl-CoA + CO2 + NAD(P)H
3-oxopropanoate
+ CoA
+
NAD(P)(+)
Bound ligand (Het Group name = NAD)
matches with 91.00% similarity
= acetyl-CoA
+ CO(2)
+ NAD(P)H
   Enzyme class 2: E.C.1.2.1.27  - Methylmalonate-semialdehyde dehydrogenase (CoA acylating).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH
2-methyl-3-oxopropanoate
+ CoA
+ H(2)O
+
NAD(+)
Bound ligand (Het Group name = NAD)
corresponds exactly
= propanoyl-CoA
+ HCO(3)(-)
+ NADH
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   3 terms 
  Biochemical function     oxidoreductase activity     4 terms