PDBsum entry 1rl8

Hydrolase/hydrolase inhibitor

PDB id: 1rl8

Contents

Protein chains

99 a.a. *

Ligands

RIT

Waters ×143

* Residue conservation analysis

PDB id:

1rl8

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of the complex of resistant strain of HIV-1 protease(v82a mutant) with ritonavir

Structure:

Protease retropepsin. Chain: a, b. Synonym: HIV-1 protease. Engineered: yes. Mutation: yes. Other_details: complexed with ritonavir

Source:

Human immunodeficiency virus 1. Organism_taxid: 11676. Gene: gag-pol. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PQS)

Resolution:

2.00Å R-factor: 0.226 R-free: 0.269

Authors:

P.Rezacova,J.Brynda,J.Sedlacek,J.Konvalinka,M.Fabry,M.Horejsi

Key ref:

P.Rezacova et al. Crystal structure of the complex of resistant strain of HIV-1 protease(V82a mutant) with ritonavir. To be published, .

Date:

25-Nov-03 Release date: 19-Apr-05

Protein chains

P03367  (POL_HV1BR) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate BRU/LAI)

Seq: 1447 a.a.

Struc: 99 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 2: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 4: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 5: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site