PDBsum entry 1r8o

Hydrolase inhibitor

PDB id: 1r8o

Contents

Protein chains

96 a.a. *

71 a.a. *

Waters ×182

* Residue conservation analysis

PDB id:

1r8o

Name:

Hydrolase inhibitor

Title:

Crystal structure of an unusual kunitz-type trypsin inhibitor from copaifera langsdorffii seeds

Structure:

Kunitz trypsin inhibitor. Chain: a. Fragment: residues 1-96. Synonym: sti. Kunitz trypsin inhibitor. Chain: b. Fragment: residues 97-167. Synonym: sti

Source:

Copaifera langsdorffii. Organism_taxid: 280048. Organism_taxid: 280048

Biol. unit:

Dimer (from PQS)

Resolution:

1.83Å R-factor: 0.170 R-free: 0.212

Authors:

S.Krauchenco,R.A.P.Nagem,J.A.Da Silva,S.Marangoni,I.Polikarpov

Key ref:

S.Krauchenco et al. (2004). Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii. Biochimie, 86, 167-172. PubMed id: 15134830 DOI: 10.1016/j.biochi.2004.03.004

Date:

27-Oct-03 Release date: 25-May-04

Protein chain

P84144  (P84144_COPLA) -

Protein chain

P84145  (P84145_COPLA) -

DOI no: 10.1016/j.biochi.2004.03.004

Biochimie 86:167-172 (2004)

PubMed id: 15134830

Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii.

S.Krauchenco, R.A.Nagem, J.A.da Silva, S.Marangoni, I.Polikarpov.

ABSTRACT

The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed.