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* Residue conservation analysis
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DOI no:
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J Mol Biol
297:1159-1170
(2000)
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PubMed id:
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The structure of Antirrhinum centroradialis protein (CEN) suggests a role as a kinase regulator.
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M.J.Banfield,
R.L.Brady.
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ABSTRACT
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Expression of the plant protein centroradialis (CEN) leads to a morphological
switch between shoot growth and the development of flower structures
(inflorescence). We have determined the crystal structure of Antirrhinum CEN to
1.9 A resolution. This structure confirms the CEN proteins as a subset of the
family of phosphatidylethanolamine-binding proteins (PEBP), as predicted from
sequence homology. Mammalian forms of PEBP have been found to act as inhibitors
of MAP kinase signalling, a central signalling cascade regulating cell
differentiation. CEN and PEBP proteins share a similar topology dominated by a
large central beta-sheet. The strong conservation of a binding pocket at one end
of this sheet which is capable of binding phosphoryl ligands, suggests the
biological effects of CEN, like PEBP, arise from the ability of this region to
form complexes with phosphorylated ligands, hence interfering with kinases and
their effectors.
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Selected figure(s)
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Figure 2.
Figure 2. (a) MOLSCRIPT [Kraulis 1991] representation of
the crystal structure of Antirrhinum CEN. The invariant
histidine (His86) in the ligand-binding site is shown in yellow
in ball-and-stick representation. The Figure was prepared with
BOBSCRIPT [Esnouf 1997], and rendered with Raster3D [Merritt and
Bacon 1997]. (b) Stereoview showing the C^a traces of the
crystal structure of Antirrhinum CEN superimposed on the
structure of human PEBP. CEN (green) and hPEBP (red lines). The
amino and carboxy termini are labelled N and C, respectively,
and the side-chain for His86 is shown yellow. The Figure was
prepared with BOBSCRIPT [Esnouf 1997].
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Figure 5.
Figure 5. The arrangement of conserved residues forming the
ligand-binding site in (a) bPEBP, (b) hPEBP and (c) CEN. Each
model is in approximately the same orientation. The location of
the bound phosphoryl group (PE) is shown with bPEBP [Serre et al
1998] in (a), and the location of the bound cacodylate ion
(labelled CAC) is shown with hPEBP in (b). Hydrogen bonds are
shown as broken magenta lines.
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The above figures are
reprinted
by permission from Elsevier:
J Mol Biol
(2000,
297,
1159-1170)
copyright 2000.
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Figures were
selected
by the author.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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A.N.Shemon,
G.L.Heil,
A.E.Granovsky,
M.M.Clark,
D.McElheny,
A.Chimon,
M.R.Rosner,
and
S.Koide
(2010).
Characterization of the Raf kinase inhibitory protein (RKIP) binding pocket: NMR-based screening identifies small-molecule ligands.
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PLoS One,
5,
e10479.
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H.Hedman,
T.Källman,
and
U.Lagercrantz
(2009).
Early evolution of the MFT-like gene family in plants.
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Plant Mol Biol,
70,
359-369.
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Y.A.Purwestri,
Y.Ogaki,
S.Tamaki,
H.Tsuji,
and
K.Shimamoto
(2009).
The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a.
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Plant Cell Physiol,
50,
429-438.
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A.Argiriou,
G.Michailidis,
and
A.S.Tsaftaris
(2008).
Characterization and expression analysis of TERMINAL FLOWER1 homologs from cultivated alloteraploid cotton (Gossypium hirsutum) and its diploid progenitors.
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J Plant Physiol,
165,
1636-1646.
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K.A.Shepard
(2007).
The molecular population genetics of shoot development in Arabidopsis thaliana.
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Genetica,
129,
19-36.
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M.J.Carmona,
M.Calonje,
and
J.M.Martínez-Zapater
(2007).
The FT/TFL1 gene family in grapevine.
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Plant Mol Biol,
63,
637-650.
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M.Minami,
O.Kureha,
M.Mori,
H.Kamitsuji,
K.Suzuki,
and
T.Irie
(2007).
Long serial analysis of gene expression for transcriptome profiling during the initiation of ligninolytic enzymes production in Phanerochaete chrysosporium.
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Appl Microbiol Biotechnol,
75,
609-618.
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W.Q.Chen,
A.Viidik,
M.Skalicky,
H.Höger,
and
G.Lubec
(2007).
Hippocampal signaling cascades are modulated in voluntary and treadmill exercise rats.
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Electrophoresis,
28,
4392-4400.
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J.H.Ahn,
D.Miller,
V.J.Winter,
M.J.Banfield,
J.H.Lee,
S.Y.Yoo,
S.R.Henz,
R.L.Brady,
and
D.Weigel
(2006).
A divergent external loop confers antagonistic activity on floral regulators FT and TFL1.
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EMBO J,
25,
605-614.
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PDB codes:
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J.Mima,
H.Fukada,
M.Nagayama,
and
M.Ueda
(2006).
Specific membrane binding of the carboxypeptidase Y inhibitor I(C), a phosphatidylethanolamine-binding protein family member.
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FEBS J,
273,
5374-5383.
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P.A.Wigge,
M.C.Kim,
K.E.Jaeger,
W.Busch,
M.Schmid,
J.U.Lohmann,
and
D.Weigel
(2005).
Integration of spatial and temporal information during floral induction in Arabidopsis.
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Science,
309,
1056-1059.
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T.Endo,
T.Shimada,
H.Fujii,
Y.Kobayashi,
T.Araki,
and
M.Omura
(2005).
Ectopic expression of an FT homolog from citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.).
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Transgenic Res,
14,
703-712.
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Y.Hanzawa,
T.Money,
and
D.Bradley
(2005).
A single amino acid converts a repressor to an activator of flowering.
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Proc Natl Acad Sci U S A,
102,
7748-7753.
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H.Chautard,
M.Jacquet,
F.Schoentgen,
N.Bureaud,
and
H.Bénédetti
(2004).
Tfs1p, a member of the PEBP family, inhibits the Ira2p but not the Ira1p Ras GTPase-activating protein in Saccharomyces cerevisiae.
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Eukaryot Cell,
3,
459-470.
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L.Sreekantan,
J.Clemens,
M.J.McKenzie,
J.R.Lenton,
S.J.Croker,
and
P.E.Jameson
(2004).
Flowering genes in Metrosideros fit a broad herbaceous model encompassing Arabidopsis and Antirrhinum.
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Physiol Plant,
121,
163-173.
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B.S.Vallée,
G.Coadou,
H.Labbé,
D.Sy,
F.Vovelle,
and
F.Schoentgen
(2003).
Peptides corresponding to the N- and C-terminal parts of PEBP are well-structured in solution: new insights into their possible interaction with partners in vivo.
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J Pept Res,
61,
47-57.
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D.M.Hickox,
G.Gibbs,
J.R.Morrison,
K.Sebire,
K.Edgar,
H.H.Keah,
K.Alter,
K.L.Loveland,
M.T.Hearn,
D.M.de Kretser,
and
M.K.O'Bryan
(2002).
Identification of a novel testis-specific member of the phosphatidylethanolamine binding protein family, pebp-2.
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Biol Reprod,
67,
917-927.
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P.C.Simister,
M.J.Banfield,
and
R.L.Brady
(2002).
The crystal structure of PEBP-2, a homologue of the PEBP/RKIP family.
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Acta Crystallogr D Biol Crystallogr,
58,
1077-1080.
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PDB code:
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K.C.Yeung,
D.W.Rose,
A.S.Dhillon,
D.Yaros,
M.Gustafsson,
D.Chatterjee,
B.McFerran,
J.Wyche,
W.Kolch,
and
J.M.Sedivy
(2001).
Raf kinase inhibitor protein interacts with NF-kappaB-inducing kinase and TAK1 and inhibits NF-kappaB activation.
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Mol Cell Biol,
21,
7207-7217.
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N.Mimida,
K.Goto,
Y.Kobayashi,
T.Araki,
J.H.Ahn,
D.Weigel,
M.Murata,
F.Motoyoshi,
and
W.Sakamoto
(2001).
Functional divergence of the TFL1-like gene family in Arabidopsis revealed by characterization of a novel homologue.
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Genes Cells,
6,
327-336.
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T.Kroslak,
T.Koch,
E.Kahl,
and
V.Höllt
(2001).
Human phosphatidylethanolamine-binding protein facilitates heterotrimeric G protein-dependent signaling.
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J Biol Chem,
276,
39772-39778.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
