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PDBsum entry 1ptk
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Hydrolase(serine proteinase)
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PDB id
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1ptk
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Contents |
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* Residue conservation analysis
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Enzyme class:
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E.C.3.4.21.64
- peptidase K.
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Reaction:
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Hydrolysis of keratin and of other proteins, with subtilisin-like specificity. Hydrolyzes peptides amides.
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J Biol Chem
268:26150-26154
(1993)
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PubMed id:
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Studies on the inhibitory action of mercury upon proteinase K.
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A.Müller,
W.Saenger.
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ABSTRACT
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In proteinase K, Cys73 is located "below" the imidazole of the active site
His69. In a 2.4-A resolution x-ray crystal structure of the complex formed
between the enzyme and HgAc2, two Hg(II) positions are found: a fully occupied
site, covalently bound to Cys73 (S gamma), which disrupts the catalytic triad
(Asp39-His69-Ser224), and a 2-fold disordered (25 and 35% occupancy),
noncovalent complexation to His72, Cys73, and Thr76 of lower affinity. The
enzyme is inhibited noncompetitively at low concentrations and competitively
above stoichiometric concentrations of Hg(II), but it retains 7% residual
activity. This can be rationalized if the molecule is flexible enough to permit
transient formation of the catalytic triad. Except for the active site, only
minor structural changes are observed upon binding of Hg(II), but the thermal
stability is reduced by 4 degrees C.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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J.J.Panek,
R.Mazzarello,
M.Novič,
and
A.Jezierska-Mazzarello
(2011).
Impact of Mercury(II) on proteinase K catalytic center: investigations via classical and Born-Oppenheimer molecular dynamics.
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Mol Divers,
15,
215-226.
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C.D.Matheson,
T.E.Marion,
S.Hayter,
N.Esau,
R.Fratpietro,
and
K.K.Vernon
(2009).
Technical note: removal of metal ion inhibition encountered during DNA extraction and amplification of copper-preserved archaeological bone using size exclusion chromatography.
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Am J Phys Anthropol,
140,
384-391.
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M.D.Rand,
C.E.Bland,
and
J.Bond
(2008).
Methylmercury activates enhancer-of-split and bearded complex genes independent of the notch receptor.
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Toxicol Sci,
104,
163-176.
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E.K.Kotlova,
N.M.Ivanova,
M.P.Yusupova,
T.L.Voyushina,
N.E.Ivanushkina,
and
G.G.Chestukhina
(2007).
Thiol-dependent serine proteinase from Paecilomyces lilacinus: purification and catalytic properties.
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Biochemistry (Mosc),
72,
117-123.
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F.Lewis,
P.Jackson,
S.Lane,
G.Coast,
and
A.M.Hanby
(2004).
Testing for HER2 in breast cancer.
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Histopathology,
45,
207-217.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
