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PDBsum entry 1ohs

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Isomerase PDB id
1ohs
Jmol
Contents
Protein chains
125 a.a. *
Ligands
5SD ×3
Waters ×419
* Residue conservation analysis
PDB id:
1ohs
Name: Isomerase
Title: Crystal structure of 5-3-ketosteroid isomerase mutant y14f/ d38n from pseudomonas testosteroni complexed with androstanedione
Structure: Steroid delta-isomerase. Chain: a, b, c, d. Synonym: delta-5-3-ketosteroid isomerase, ketosteroid isomerase. Engineered: yes. Mutation: yes
Source: Pseudomonas testosteroni. Organism_taxid: 285. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Biol. unit: Dimer (from PDB file)
Resolution:
1.7Å     R-factor:   0.219     R-free:   0.249
Authors: M.Byun,M.-S.Kim,B.-H.Oh
Key ref: M.Byun et al. Crystal structure of 5-3-Ketosteroid isomerase mutant y14f/d38n from pseudomonas testosteroni complexed with androstanedione. To be published, .
Date:
30-May-03     Release date:   31-Mar-05    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00947  (SDIS_COMTE) -  Steroid Delta-isomerase
Seq:
Struc:
125 a.a.
125 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.5.3.3.1  - Steroid Delta-isomerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid
3-oxo-Delta(5)-steroid
Bound ligand (Het Group name = 5SD)
matches with 86.00% similarity
= 3-oxo-Delta(4)-steroid
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   1 term 
  Biological process     transport   3 terms 
  Biochemical function     isomerase activity     2 terms