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* Residue conservation analysis
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Enzyme class:
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Chain A:
E.C.3.4.21.4
- trypsin.
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Reaction:
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Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
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J Mol Biol
229:1022-1036
(1993)
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PubMed id:
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Refined 1.6 A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex.
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Q.Huang,
S.Liu,
Y.Tang.
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ABSTRACT
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The crystal structure of the complex formed by porcine beta-trypsin with the
MCTI-A inhibitor (Momordica charantia, Linn. Cucurbitaceae) has been determined
at 1.6 A resolution using the molecular replacement method. The sequence of
MCTI-A was determined by recognizing the electron density, and shows that MCTI-A
is a member of the squash family of trypsin inhibitors. We report the first
high-resolution structure of porcine beta-trypsin. Detailed comparisons have
been made on the overall structure, solvent structure and active-site geometries
between this complex and bovine beta-trypsin and its complexes. On the basis of
our results, we discuss the interaction patterns between inhibitor and trypsin.
Unlike other complex structures formed by bovine trypsin with inhibitors, no
out-of-plane distortion around the inhibitor's scissible peptide was observed.
The role of the trypsin catalytic triad is also discussed on the basis of this
structure.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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Y.Zhou,
and
Y.Zhang
(2011).
Serine protease acylation proceeds with a subtle re-orientation of the histidine ring at the tetrahedral intermediate.
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Chem Commun (Camb),
47,
1577-1579.
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A.Heitz,
O.Avrutina,
D.Le-Nguyen,
U.Diederichsen,
J.F.Hernandez,
J.Gracy,
H.Kolmar,
and
L.Chiche
(2008).
Knottin cyclization: Impact on Structure and Dynamics.
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BMC Struct Biol,
8,
54.
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J.W.Torrance,
M.W.Macarthur,
and
J.M.Thornton
(2008).
Evolution of binding sites for zinc and calcium ions playing structural roles.
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Proteins,
71,
813-830.
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A.A.Gorfe,
B.O.Brandsdal,
H.K.Leiros,
R.Helland,
and
A.O.Smalås
(2000).
Electrostatics of mesophilic and psychrophilic trypsin isoenzymes: qualitative evaluation of electrostatic differences at the substrate binding site.
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Proteins,
40,
207-217.
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H.K.Leiros,
N.P.Willassen,
and
A.O.Smalås
(2000).
Structural comparison of psychrophilic and mesophilic trypsins. Elucidating the molecular basis of cold-adaptation.
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Eur J Biochem,
267,
1039-1049.
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K.Kamei,
S.Sato,
N.Hamato,
R.Takano,
K.Ohshima,
R.Yamamoto,
T.Nishino,
H.Kato,
and
S.Hara
(2000).
Effect of P(2)' site tryptophan and P(20)' site deletion of Momordica charantia trypsin inhibitor II on inhibition of proteinases.
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Biochim Biophys Acta,
1480,
6.
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H.M.Murthy,
S.Clum,
and
R.Padmanabhan
(1999).
Dengue virus NS3 serine protease. Crystal structure and insights into interaction of the active site with substrates by molecular modeling and structural analysis of mutational effects.
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J Biol Chem,
274,
5573-5580.
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PDB code:
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R.Helland,
G.I.Berglund,
J.Otlewski,
W.Apostoluk,
O.A.Andersen,
N.P.Willassen,
and
A.O.Smalås
(1999).
High-resolution structures of three new trypsin-squash-inhibitor complexes: a detailed comparison with other trypsins and their complexes.
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Acta Crystallogr D Biol Crystallogr,
55,
139-148.
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PDB codes:
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R.Recacha,
M.Carson,
M.J.Costanzo,
B.Maryanoff,
L.J.DeLucas,
and
D.Chattopadhyay
(1999).
Structure of the RWJ-51084-bovine pancreatic beta-trypsin complex at 1.8 A.
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Acta Crystallogr D Biol Crystallogr,
55,
1785-1791.
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PDB code:
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M.T.Stubbs,
R.Morenweiser,
J.Stürzebecher,
M.Bauer,
W.Bode,
R.Huber,
G.P.Piechottka,
G.Matschiner,
C.P.Sommerhoff,
H.Fritz,
and
E.A.Auerswald
(1997).
The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin. Implications for the structure of human mast cell tryptase and its inhibition.
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J Biol Chem,
272,
19931-19937.
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PDB code:
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S.Di Marco,
and
J.P.Priestle
(1997).
Structure of the complex of leech-derived tryptase inhibitor (LDTI) with trypsin and modeling of the LDTI-tryptase system.
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Structure,
5,
1465-1474.
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PDB code:
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J.Janin
(1995).
Elusive affinities.
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Proteins,
21,
30-39.
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S.L.Lin,
and
R.Nussinov
(1995).
A disulphide-reinforced structural scaffold shared by small proteins with diverse functions.
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Nat Struct Biol,
2,
835-837.
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A.O.Smalås,
E.S.Heimstad,
A.Hordvik,
N.P.Willassen,
and
R.Male
(1994).
Cold adaption of enzymes: structural comparison between salmon and bovine trypsins.
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Proteins,
20,
149-166.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
