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PDBsum entry 1lx7

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protein Protein-protein interface(s) links
Transferase PDB id
1lx7
Jmol
Contents
Protein chains
250 a.a. *
221 a.a. *
Waters ×361
* Residue conservation analysis
PDB id:
1lx7
Name: Transferase
Title: Structure of e. Coli uridine phosphorylase at 2.0a
Structure: Uridine phosphorylase. Chain: a, b. Synonym: udrpase. Engineered: yes
Source: Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: t7 expression system
Biol. unit: Hexamer (from PDB file)
Resolution:
2.00Å     R-factor:   0.182     R-free:   0.215
Authors: T.Burling,J.A.Buglino,R.Kniewel,T.Chadna,A.Beckwith,C.D.Lima S.K.Burley,New York Sgx Research Center For Structural Geno (Nysgxrc)
Key ref:
F.T.Burling et al. (2003). Structure of Escherichia coli uridine phosphorylase at 2.0 A. Acta Crystallogr D Biol Crystallogr, 59, 73-76. PubMed id: 12499542
Date:
04-Jun-02     Release date:   12-Jun-02    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P12758  (UDP_ECOLI) -  Uridine phosphorylase
Seq:
Struc:
253 a.a.
250 a.a.
Protein chain
Pfam   ArchSchema ?
P12758  (UDP_ECOLI) -  Uridine phosphorylase
Seq:
Struc:
253 a.a.
221 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.2.4.2.3  - Uridine phosphorylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Uridine
+ phosphate
= uracil
+ alpha-D-ribose 1-phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   3 terms 
  Biological process     UMP salvage   4 terms 
  Biochemical function     catalytic activity     6 terms  

 

 
    reference    
 
 
Acta Crystallogr D Biol Crystallogr 59:73-76 (2003)
PubMed id: 12499542  
 
 
Structure of Escherichia coli uridine phosphorylase at 2.0 A.
F.T.Burling, R.Kniewel, J.A.Buglino, T.Chadha, A.Beckwith, C.D.Lima.
 
  ABSTRACT  
 
The 2.0 A crystal structure has been determined for Escherichia coli uridine phosphorylase (UP), an essential enzyme in nucleotide biosynthesis that catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose-1-phosphate and uracil. The structure determination of two independent monomers in the asymmetric unit revealed the residue composition and atomic details of the apo configurations of each active site. The native hexameric UP enzyme was revealed by applying threefold crystallographic symmetry to the contents of the asymmetric unit. The 2.0 A model reveals a closer structural relationship to other nucleotide phosphorylase enzymes than was previously appreciated.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20364833 D.Paul, S.E.O'Leary, K.Rajashankar, W.Bu, A.Toms, E.C.Settembre, J.M.Sanders, T.P.Begley, and S.E.Ealick (2010).
Glycal formation in crystals of uridine phosphorylase.
  Biochemistry, 49, 3499-3509.
PDB codes: 3ku4 3kuk 3kvr 3kvv 3kvy
20856879 T.P.Roosild, and S.Castronovo (2010).
Active site conformational dynamics in human uridine phosphorylase 1.
  PLoS One, 5, e12741.
PDB code: 3nbq
19291308 T.P.Roosild, S.Castronovo, M.Fabbiani, and G.Pizzorno (2009).
Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy.
  BMC Struct Biol, 9, 14.
PDB codes: 3eue 3euf
14557261 F.Vincent, D.Yates, E.Garman, G.J.Davies, and J.A.Brannigan (2004).
The three-dimensional structure of the N-acetylglucosamine-6-phosphate deacetylase, NagA, from Bacillus subtilis: a member of the urease superfamily.
  J Biol Chem, 279, 2809-2816.
PDB codes: 1un7 2vhl
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.