PDBsum entry 1k4g

Go to PDB code: 
protein ligands metals links
Transferase PDB id
Protein chain
372 a.a. *
Waters ×382
* Residue conservation analysis
PDB id:
Name: Transferase
Title: Crystal structure of tRNA-guanine transglycosylase (tgt) com with 2,6-diamino-8-(1h-imidazol-2-ylsulfanylmethyl)-3h-quin one
Structure: tRNA-guanine transglycosylase. Chain: a. Synonym: tgt, queuine tRNA-ribosyltransferase, guanine inse enzyme. Engineered: yes
Source: Zymomonas mobilis. Organism_taxid: 542. Expressed in: escherichia coli. Expression_system_taxid: 562
Biol. unit: Dimer (from PQS)
1.70Å     R-factor:   0.199     R-free:   0.241
Authors: R.Brenk,E.A.Meyer,R.K.Castellano,M.Furler,M.T.Stubbs,G.Klebe F.Diederich
Key ref: E.A.Meyer et al. (2002). De novo design, synthesis, and in vitro evaluation of inhibitors for prokaryotic tRNA-guanine transglycosylase: a dramatic sulfur effect on binding affinity. Chembiochem, 3, 250-253. PubMed id: 11921407 DOI: 10.1002/1439-7633(20020301)3:2/3<250::AID-CBIC250>3.0.CO;2-J
08-Oct-01     Release date:   24-Apr-02    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P28720  (TGT_ZYMMO) -  Queuine tRNA-ribosyltransferase
386 a.a.
372 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.  - tRNA-guanine(34) transglycosylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
1. Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine
2. Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7- carbaguanine34 in tRNA + guanine
Guanine(34) in tRNA
Bound ligand (Het Group name = AIQ)
matches with 50.00% similarity
= queuosine(34) in tRNA
+ guanine
Guanine(34) in tRNA
Bound ligand (Het Group name = AIQ)
matches with 50.00% similarity
= 7-aminomethyl-7- carbaguanine(34) in tRNA
+ guanine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     tRNA processing   3 terms 
  Biochemical function     transferase activity     4 terms  


DOI no: 10.1002/1439-7633(20020301)3:2/3<250::AID-CBIC250>3.0.CO;2-J Chembiochem 3:250-253 (2002)
PubMed id: 11921407  
De novo design, synthesis, and in vitro evaluation of inhibitors for prokaryotic tRNA-guanine transglycosylase: a dramatic sulfur effect on binding affinity.
E.A.Meyer, R.Brenk, R.K.Castellano, M.Furler, G.Klebe, F.Diederich.
No abstract given.


Literature references that cite this PDB file's key reference

  PubMed id Reference
17441158 A.Goel, F.V.Singh, M.Dixit, D.Verma, R.Raghunandan, and P.R.Maulik (2007).
Highly efficient non-palladium-catalyzed controlled synthesis and X-ray analysis of functionalized 1,2-diaryl-, 1,2,3-triaryl-, and 1,2,3,4-tetraarylbenzenes.
  Chem Asian J, 2, 239-247.  
16206323 B.Stengl, K.Reuter, and G.Klebe (2005).
Mechanism and substrate specificity of tRNA-guanine transglycosylases (TGTs): tRNA-modifying enzymes from the three different kingdoms of life share a common catalytic mechanism.
  Chembiochem, 6, 1926-1939.  
15146480 O.Kraemer, I.Hazemann, A.D.Podjarny, and G.Klebe (2004).
Virtual screening for inhibitors of human aldose reductase.
  Proteins, 55, 814-823.  
15239063 S.C.Miller, and T.J.Mitchison (2004).
Synthesis and phenotypic screening of a Guanine-mimetic library.
  Chembiochem, 5, 1010-1012.  
15239059 S.Sahli, B.Stump, T.Welti, D.Blum-Kaelin, J.D.Aebi, C.Oefner, H.J.Böhm, and F.Diederich (2004).
Structure-based design, synthesis, and in vitro evaluation of nonpeptidic neprilysin inhibitors.
  Chembiochem, 5, 996.  
14523925 R.Brenk, M.T.Stubbs, A.Heine, K.Reuter, and G.Klebe (2003).
Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design.
  Chembiochem, 4, 1066-1077.
PDB codes: 1ozm 1ozq 1p0b 1p0d 1p0e
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.