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PDBsum entry 1gs3

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protein ligands links
Isomerase PDB id
1gs3
Jmol
Contents
Protein chain
123 a.a. *
Ligands
EQU
Waters ×38
* Residue conservation analysis
PDB id:
1gs3
Name: Isomerase
Title: High resolution crystal structure of pi delta-5-3-ketosteroid isomerase mutants y30f/y55f/y115f/ d38n (y32f/y57f/y119f/d40n, pi numbering)complexed with equilenin at 2.1 a resolution
Structure: Steroid delta-isomerase. Chain: a. Synonym: delta-5-3-ketosteroid isomerase. Engineered: yes. Mutation: yes
Source: Pseudomonas putida. Organism_taxid: 303. Strain: biotype b. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Biol. unit: Dimer (from PDB file)
Resolution:
2.1Å     R-factor:   0.212     R-free:   0.275
Authors: S.Shin,N.-C.Ha,B.-H.Oh
Key ref: G.Choi et al. Contribution of a low-Barrier hydrogen bond to catalysis by delta-5-3-Ketosteroid isomerase is not extremely high compared to that of an ordinary hydrogen bond. Low-Barrier hydrogen bond of pi ksi. To be published, .
Date:
27-Dec-01     Release date:   23-Jan-03    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P07445  (SDIS_PSEPU) -  Steroid Delta-isomerase
Seq:
Struc:
131 a.a.
123 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.5.3.3.1  - Steroid Delta-isomerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid
3-oxo-Delta(5)-steroid
Bound ligand (Het Group name = EQU)
matches with 90.00% similarity
= 3-oxo-Delta(4)-steroid
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   1 term 
  Biological process     transport   3 terms 
  Biochemical function     isomerase activity     2 terms