PDBsum entry 1gpw

Lyase/transferase

PDB id: 1gpw

Contents

Protein chains

253 a.a. *

201 a.a. *

Ligands

PO4 ×6

Waters ×459

* Residue conservation analysis

PDB id:

1gpw

Name:

Lyase/transferase

Title:

Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex.

Structure:

Hisf protein. Chain: a, c, e. Engineered: yes. Mutation: yes. Amidotransferase hish. Chain: b, d, f. Engineered: yes

Source:

Thermotoga maritima. Organism_taxid: 2336. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: w3110 delta-trpea2.

Biol. unit:

Hetero-Dimer (from PDB file)

Resolution:

2.40Å R-factor: 0.226 R-free: 0.290

Authors:

M.Walker,S.Beismann-Driemeyer,R.Sterner,M.Wilmanns

Date:

12-Nov-01 Release date: 10-Feb-02

Protein chains

Q9X0C6  (HIS6_THEMA) -  Imidazole glycerol phosphate synthase subunit HisF from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

Seq: 253 a.a.

Struc: 253 a.a.*

Protein chains

Q9X0C8  (HIS5_THEMA) -  Imidazole glycerol phosphate synthase subunit HisH from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

Seq: 201 a.a.

Struc: 201 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D, E, F: E.C.4.3.2.10 - imidazole glycerol-phosphate synthase.
• Reaction: 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta- D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol- 4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D- ribosyl)imidazole-4-carboxamide + L-glutamate + H⁺
• Enzyme class 3: Chains B, D, F: E.C.3.5.1.2 - glutaminase.
• Reaction: L-glutamine + H2O = L-glutamate + NH4⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site