PDBsum entry 1fli

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protein links
Transcription/DNA PDB id
Protein chain
98 a.a. *
* Residue conservation analysis
PDB id:
Name: Transcription/DNA
Title: DNA-binding domain of fli-1
Structure: Fli-1. Chain: a. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: t7. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 30 models
Authors: H.Liang,X.Mao,E.T.Olejniczak,D.G.Nettesheim,L.Yu, R.P.Meadows,C.B.Thompson,S.W.Fesik
Key ref: H.Liang et al. (1994). Solution structure of the ets domain of Fli-1 when bound to DNA. Nat Struct Biol, 1, 871-875. PubMed id: 7773776
15-Sep-94     Release date:   15-Sep-95    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
Q01543  (FLI1_HUMAN) -  Friend leukemia integration 1 transcription factor
452 a.a.
98 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     regulation of transcription, DNA-dependent   1 term 
  Biochemical function     sequence-specific DNA binding transcription factor activity     2 terms  


Nat Struct Biol 1:871-875 (1994)
PubMed id: 7773776  
Solution structure of the ets domain of Fli-1 when bound to DNA.
H.Liang, X.Mao, E.T.Olejniczak, D.G.Nettesheim, L.Yu, R.P.Meadows, C.B.Thompson, S.W.Fesik.
Members of the ets family of transcription factors share a conserved DNA-binding domain, the ets domain. By using multidimensional NMR, we have determined the structure of the ets domain of human Fli-1 in the DNA-bound form. It consists of three alpha-helices and a four-stranded beta-sheet, similar to structures of the class of helix-turn-helix DNA binding proteins first found in the catabolite activator protein of Escherichia coli. NMR and mutagenesis experiments suggest that in comparison to structurally related proteins, the ets domain uses a new variation of the helix-turn-helix motif for binding to DNA.

Literature references that cite this PDB file's key reference

  PubMed id Reference
20522024 T.Shimokawa, S.Nunomura, Y.Enomoto, and C.Ra (2010).
Amino acid residues in the beta3 strand and subsequent loop of the conserved ETS domain that mediate basic leucine zipper (bZIP) recruitment and potentially distinguish functional attributes of Ets proteins.
  Biochem J, 430, 129-139.  
20079749 V.B.Agarkar, N.D.Babayeva, P.J.Wilder, A.Rizzino, and T.H.Tahirov (2010).
Crystal structure of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA.
  J Mol Biol, 397, 278-289.
PDB code: 3jtg
18420658 M.R.Dyson, R.L.Perera, S.P.Shadbolt, L.Biderman, K.Bromek, N.V.Murzina, and J.McCafferty (2008).
Identification of soluble protein fragments by gene fragmentation and genetic selection.
  Nucleic Acids Res, 36, e51.  
18500345 S.J.Loughran, E.A.Kruse, D.F.Hacking, Graaf, C.D.Hyland, T.A.Willson, K.J.Henley, S.Ellis, A.K.Voss, D.Metcalf, D.J.Hilton, W.S.Alexander, and B.T.Kile (2008).
The transcription factor Erg is essential for definitive hematopoiesis and the function of adult hematopoietic stem cells.
  Nat Immunol, 9, 810-819.  
17148437 J.L.Kopp, P.J.Wilder, M.Desler, L.Kinarsky, and A.Rizzino (2007).
Different domains of the transcription factor ELF3 are required in a promoter-specific manner and multiple domains control its binding to DNA.
  J Biol Chem, 282, 3027-3041.  
  19279707 T.Kiyota, A.Kato, and Y.Kato (2007).
Ets-1 regulates radial glia formation during vertebrate embryogenesis.
  Organogenesis, 3, 93.  
16242051 N.R.McAllister, and S.L.Lessnick (2005).
The potential for molecular therapeutic targets in Ewing's sarcoma.
  Curr Treat Options Oncol, 6, 461-471.  
15798196 W.Hu, A.S.Philips, J.C.Kwok, M.Eisbacher, and B.H.Chong (2005).
Identification of nuclear import and export signals within Fli-1: roles of the nuclear import signals in Fli-1-dependent activation of megakaryocyte-specific promoters.
  Mol Cell Biol, 25, 3087-3108.  
14570912 S.Ano, R.Pereira, M.Pironin, I.Lesault, C.Milley, I.Lebigot, C.T.Quang, and J.Ghysdael (2004).
Erythroblast transformation by FLI-1 depends upon its specific DNA binding and transcriptional activation properties.
  J Biol Chem, 279, 2993-3002.  
12724402 M.Eisbacher, M.L.Holmes, A.Newton, P.J.Hogg, L.M.Khachigian, M.Crossley, and B.H.Chong (2003).
Protein-protein interaction between Fli-1 and GATA-1 mediates synergistic expression of megakaryocyte-specific genes through cooperative DNA binding.
  Mol Cell Biol, 23, 3427-3441.  
12757758 P.E.Shaw, and J.Saxton (2003).
Ternary complex factors: prime nuclear targets for mitogen-activated protein kinases.
  Int J Biochem Cell Biol, 35, 1210-1226.  
11841229 G.M.Poon, P.Gross, and R.B.Macgregor (2002).
The sequence-specific association of the ETS domain of murine PU.1 with DNA exhibits unusual energetics.
  Biochemistry, 41, 2361-2371.  
12402362 P.P.Liu, Y.C.Chen, C.Li, Y.H.Hsieh, S.W.Chen, S.H.Chen, W.Y.Jeng, and W.J.Chuang (2002).
Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a).
  Proteins, 49, 543-553.
PDB code: 1jxs
11553628 S.M.Welford, S.P.Hebert, B.Deneen, A.Arvand, and C.T.Denny (2001).
DNA binding domain-independent pathways are involved in EWS/FLI1-mediated oncogenesis.
  J Biol Chem, 276, 41977-41984.  
10636872 V.Drewett, S.Muller, J.Goodall, and P.E.Shaw (2000).
Dimer formation by ternary complex factor ELK-1.
  J Biol Chem, 275, 1757-1762.  
10410794 C.Wolberger (1999).
Multiprotein-DNA complexes in transcriptional regulation.
  Annu Rev Biophys Biomol Struct, 28, 29-56.  
  10346815 H.E.Xu, M.A.Rould, W.Xu, J.A.Epstein, R.L.Maas, and C.O.Pabo (1999).
Crystal structure of the human Pax6 paired domain-DNA complex reveals specific roles for the linker region and carboxy-terminal subdomain in DNA binding.
  Genes Dev, 13, 1263-1275.
PDB code: 6pax
  10780882 A.G.Bassuk, K.P.Barton, R.T.Anandappa, M.M.Lu, and J.M.Leiden (1998).
Expression pattern of the Ets-related transcription factor Elf-1.
  Mol Med, 4, 392-401.  
9461436 A.H.Batchelor, D.E.Piper, la Brousse, S.L.McKnight, and C.Wolberger (1998).
The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA.
  Science, 279, 1037-1041.
PDB code: 1awc
10384327 B.J.Graves, D.O.Cowley, T.L.Goetz, J.M.Petersen, M.D.Jonsen, and M.E.Gillespie (1998).
Autoinhibition as a transcriptional regulatory mechanism.
  Cold Spring Harb Symp Quant Biol, 63, 621-629.  
9794820 C.Wolberger (1998).
Combinatorial transcription factors.
  Curr Opin Genet Dev, 8, 552-559.  
  18521227 H.Kovar (1998).
Progress in the molecular biology of ewing tumors.
  Sarcoma, 2, 3.  
9565750 J.P.Schneider, A.Lombardi, and W.F.DeGrado (1998).
Analysis and design of three-stranded coiled coils and three-helix bundles.
  Fold Des, 3, R29-R40.  
9546211 K.R.Ely, and R.Kodandapani (1998).
Ankyrin(g) ETS domains to DNA.
  Nat Struct Biol, 5, 255-259.  
9497343 Y.Chinenov, T.Schmidt, X.Y.Yang, and M.E.Martin (1998).
Identification of redox-sensitive cysteines in GA-binding protein-alpha that regulate DNA binding and heterodimerization.
  J Biol Chem, 273, 6203-6209.  
9734357 Y.Mo, B.Vaessen, K.Johnston, and R.Marmorstein (1998).
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
  Mol Cell, 2, 201-212.
PDB codes: 1bc7 1bc8
9570133 A.D.Sharrocks, A.L.Brown, Y.Ling, and P.R.Yates (1997).
The ETS-domain transcription factor family.
  Int J Biochem Cell Biol, 29, 1371-1387.  
9009269 C.Jousset, C.Carron, A.Boureux, C.T.Quang, C.Oury, I.Dusanter-Fourt, M.Charon, J.Levin, O.Bernard, and J.Ghysdael (1997).
A domain of TEL conserved in a subset of ETS proteins defines a specific oligomerization interface essential to the mitogenic properties of the TEL-PDGFR beta oncoprotein.
  EMBO J, 16, 69-82.  
9241421 G.Patikoglou, and S.K.Burley (1997).
Eukaryotic transcription factor-DNA complexes.
  Annu Rev Biophys Biomol Struct, 26, 289-325.  
9336459 J.M.Andreoli, S.I.Jang, E.Chung, C.M.Coticchia, P.M.Steinert, and N.G.Markova (1997).
The expression of a novel, epithelium-specific ets transcription factor is restricted to the most differentiated layers in the epidermis.
  Nucleic Acids Res, 25, 4287-4295.  
9083114 R.M.Xu, C.Koch, Y.Liu, J.R.Horton, D.Knapp, K.Nasmyth, and X.Cheng (1997).
Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis.
  Structure, 5, 349-358.
PDB code: 1bm8
8768898 A.J.Wand, and S.W.Englander (1996).
Protein complexes studied by NMR spectroscopy.
  Curr Opin Biotechnol, 7, 403-408.  
8798534 F.Pio, R.Kodandapani, C.Z.Ni, W.Shepard, M.Klemsz, S.R.McKercher, R.A.Maki, and K.R.Ely (1996).
New insights on DNA recognition by ets proteins from the crystal structure of the PU.1 ETS domain-DNA complex.
  J Biol Chem, 271, 23329-23337.  
  8745408 J.J.Skalicky, L.W.Donaldson, J.M.Petersen, B.J.Graves, and L.P.McIntosh (1996).
Structural coupling of the inhibitory regions flanking the ETS domain of murine Ets-1.
  Protein Sci, 5, 296-309.  
  8598195 L.W.Donaldson, J.M.Petersen, B.J.Graves, and L.P.McIntosh (1996).
Solution structure of the ETS domain from murine Ets-1: a winged helix-turn-helix DNA binding motif.
  EMBO J, 15, 125-134.
PDB codes: 1etc 1etd
  8628272 M.D.Jonsen, J.M.Petersen, Q.P.Xu, and B.J.Graves (1996).
Characterization of the cooperative function of inhibitory sequences in Ets-1.
  Mol Cell Biol, 16, 2065-2073.  
  8668149 P.Shore, A.J.Whitmarsh, R.Bhaskaran, R.J.Davis, J.P.Waltho, and A.D.Sharrocks (1996).
Determinants of DNA-binding specificity of ETS-domain transcription factors.
  Mol Cell Biol, 16, 3338-3349.  
7648395 A.G.Bassuk, and J.M.Leiden (1995).
A direct physical association between ETS and AP-1 transcription factors in normal human T cells.
  Immunity, 3, 223-237.  
7631419 D.Landsman, and A.P.Wolffe (1995).
Common sequence and structural features in the heat-shock factor and Ets families of DNA-binding domains.
  Trends Biochem Sci, 20, 225-226.  
7592633 F.Pio, C.Z.Ni, R.S.Mitchell, J.Knight, S.McKercher, M.Klemsz, A.Lombardo, R.A.Maki, and K.R.Ely (1995).
Co-crystallization of an ETS domain (PU.1) in complex with DNA. Engineering the length of both protein and oligonucleotide.
  J Biol Chem, 270, 24258-24263.  
7667887 M.B.Swindells (1995).
Identification of a common fold in the replication terminator protein suggests a possible mode for DNA binding.
  Trends Biochem Sci, 20, 300-302.  
8521493 M.H.Werner, M.Clore, C.L.Fisher, R.J.Fisher, L.Trinh, J.Shiloach, and A.M.Gronenborn (1995).
The solution structure of the human ETS1-DNA complex reveals a novel mode of binding and true side chain intercalation.
  Cell, 83, 761-771.
PDB codes: 1stt 1stw
7749979 P.Ernst, and S.T.Smale (1995).
Combinatorial regulation of transcription II: The immunoglobulin mu heavy chain gene.
  Immunity, 2, 427-438.  
  7609063 W.Sun, B.J.Graves, and N.A.Speck (1995).
Transactivation of the Moloney murine leukemia virus and T-cell receptor beta-chain enhancers by cbf and ets requires intact binding sites for both proteins.
  J Virol, 69, 4941-4949.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.