PDBsum entry 1ahw

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Complex (immunoglobulin/tissue factor) PDB id
Protein chains
214 a.a. *
214 a.a. *
200 a.a. *
* Residue conservation analysis
PDB id:
Name: Complex (immunoglobulin/tissue factor)
Title: A complex of extracellular domain of tissue factor with an inhibitory fab (5g9)
Structure: Immunoglobulin fab 5g9 (light chain). Chain: a, d. Fragment: light chain residues 1 - 214. Synonym: fab, fab light chain, fab heavy chain. Immunoglobulin fab 5g9 (heavy chain). Chain: b, e. Fragment: heavy chain residues 1 - 214. Synonym: fab, fab light chain, fab heavy chain. Tissue factor.
Source: Mus musculus. House mouse. Organism_taxid: 10090. Cell_line: bl21. Organ: blood. Tissue: blood. Homo sapiens. Human. Organism_taxid: 9606.
Biol. unit: Trimer (from PQS)
3.00Å     R-factor:   0.217     R-free:   0.285
Authors: M.Huang,R.Syed,E.A.Stura,M.J.Stone,R.S.Stefanko,W.Ruf, T.S.Edgington,I.A.Wilson
Key ref:
M.Huang et al. (1998). The mechanism of an inhibitory antibody on TF-initiated blood coagulation revealed by the crystal structures of human tissue factor, Fab 5G9 and TF.G9 complex. J Mol Biol, 275, 873-894. PubMed id: 9480775 DOI: 10.1006/jmbi.1997.1512
10-Apr-97     Release date:   25-Feb-98    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
P01837  (IGKC_MOUSE) -  Ig kappa chain C region
106 a.a.
214 a.a.
Protein chains
X5J519  (X5J519_MOUSE) -  IgM heavy chain VDJ region (Fragment)
107 a.a.
214 a.a.*
Protein chains
Pfam   ArchSchema ?
P13726  (TF_HUMAN) -  Tissue factor
295 a.a.
200 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 6 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     plasma membrane   2 terms 
  Biological process     blood coagulation   1 term 
  Biochemical function     antigen binding     1 term  


DOI no: 10.1006/jmbi.1997.1512 J Mol Biol 275:873-894 (1998)
PubMed id: 9480775  
The mechanism of an inhibitory antibody on TF-initiated blood coagulation revealed by the crystal structures of human tissue factor, Fab 5G9 and TF.G9 complex.
M.Huang, R.Syed, E.A.Stura, M.J.Stone, R.S.Stefanko, W.Ruf, T.S.Edgington, I.A.Wilson.
The tissue factor (TF)-initiated blood coagulation protease cascade can be greatly inhibited in vivo by a potent anti-human-TF monoclonal antibody, 5G9. This antibody binds the carboxyl module of the extracellular domain of TF with a nanomolar binding constant and inhibits the formation of the TF.VIIa.X ternary initiation complex. We have determined the crystal structures of the extra-cellular modules of human TF, Fab 5G9, and their complex (TF.5G9) to 2.4 A, 2. 5 A, and 3.0 A, respectively, and measured the apparent inhibition constants of 5G9 on a panel of TF mutants. In our unliganded TF structure, a 7 degrees change in the relative orientation between the D1 and D2 modules was observed when compared with other published TF structures. Comparison of the free and bound Fab 5G9 indicates that small segmental and side chain variation of the antibody complementarity determining regions occurred on complexation with TF. The antibody-antigen recognition involves 18 TF antigen residues and 19 Fab residues from six CDR with one of the largest buried surface areas seen to date. A combination of structural and mutagenesis data indicate that Tyr156, Lys169, Arg200, and Lys201 play the major role in the antibody recognition. The TF. 5G9 structure provides insights into the mechanism by which the antibody 5G9 inhibits formation of the TF.VIIa.X ternary complex.
  Selected figure(s)  
Figure 6.
Figure 6. Interactions in the TF·5G9 interface. Top stereoview shows the overall orientation and the interaction between some β-strands (labeled in green) of the D2 module of TF (green) with the CDRs of Fab 5G9 (blue and pale blue for heavy and light chains); bottom stereoview shows all 15 hydrogen bonds (Table 5) and all residues (labeled in black for Fab 5G9 and green for TF) involved in the TF-5G9 interaction (Table 3).
Figure 10.
  The above figures are reprinted by permission from Elsevier: J Mol Biol (1998, 275, 873-894) copyright 1998.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21481778 E.Karaca, and A.M.Bonvin (2011).
A multidomain flexible docking approach to deal with large conformational changes in the modeling of biomolecular complexes.
  Structure, 19, 555-565.  
20232097 A.Malik, A.Firoz, V.Jha, E.Sunderasan, and S.Ahmad (2010).
Modeling the three-dimensional structures of an unbound single-chain variable fragment (scFv) and its hypothetical complex with a Corynespora cassiicola toxin, cassiicolin.
  J Mol Model, 16, 1883-1893.  
  20062929 J.A.Jiao, A.B.Kelly, U.M.Marzec, E.Nieves, J.Acevedo, M.Burkhardt, A.Edwards, X.Y.Zhu, P.A.Chavaillaz, A.Wong, J.L.Wong, J.O.Egan, D.Taylor, P.R.Rhode, and H.C.Wong (2010).
Inhibition of acute vascular thrombosis in chimpanzees by an anti-human tissue factor antibody targeting the factor X binding site.
  Thromb Haemost, 103, 224-233.  
20704569 R.Ganesan, C.Eigenbrot, and D.Kirchhofer (2010).
Structural and mechanistic insight into how antibodies inhibit serine proteases.
  Biochem J, 430, 179-189.  
19361425 C.E.Leysath, A.F.Monzingo, J.A.Maynard, J.Barnett, G.Georgiou, B.L.Iverson, and J.D.Robertus (2009).
Crystal structure of the engineered neutralizing antibody M18 complexed to domain 4 of the anthrax protective antigen.
  J Mol Biol, 387, 680-693.
PDB codes: 3esu 3esv 3et9 3etb
18514224 C.J.Farady, P.F.Egea, E.L.Schneider, M.R.Darragh, and C.S.Craik (2008).
Structure of an Fab-protease complex reveals a highly specific non-canonical mechanism of inhibition.
  J Mol Biol, 380, 351-360.
PDB code: 3bn9
18297283 C.Li, M.E.Collier, G.A.Frentzou, J.Greenman, and C.Ettelaie (2008).
Investigation of the mechanisms of tissue factor-mediated evasion of tumour cells from cellular cytotoxicity.
  Cancer Immunol Immunother, 57, 1347-1355.  
18005986 J.E.Lee, A.Kuehne, D.M.Abelson, M.L.Fusco, M.K.Hart, and E.O.Saphire (2008).
Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain.
  J Mol Biol, 375, 202-216.
PDB code: 2qhr
18088348 N.Mackman (2008).
Tissue-specific hemostasis: role of tissue factor.
  J Thromb Haemost, 6, 303-305.  
18234071 V.Moreau, C.Fleury, D.Piquer, C.Nguyen, N.Novali, S.Villard, D.Laune, C.Granier, and F.Molina (2008).
PEPOP: computational design of immunogenic peptides.
  BMC Bioinformatics, 9, 71.  
18083189 Y.C.Kim, and G.Hummer (2008).
Coarse-grained models for simulations of multiprotein complexes: application to ubiquitin binding.
  J Mol Biol, 375, 1416-1433.  
17613528 H.H.Versteeg, and W.Ruf (2007).
Tissue factor coagulant function is enhanced by protein-disulfide isomerase independent of oxidoreductase activity.
  J Biol Chem, 282, 25416-25424.  
17425666 M.Hoffman, C.M.Colina, A.G.McDonald, G.M.Arepally, L.Pedersen, and D.M.Monroe (2007).
Tissue factor around dermal vessels has bound factor VII in the absence of injury.
  J Thromb Haemost, 5, 1403-1408.  
17712773 P.Scheerer, A.Kramer, L.Otte, M.Seifert, H.Wessner, C.Scholz, N.Krauss, J.Schneider-Mergener, and W.Höhne (2007).
Structure of an anti-cholera toxin antibody Fab in complex with an epitope-derived D-peptide: a case of polyspecific recognition.
  J Mol Recognit, 20, 263-274.
PDB code: 1zea
16959886 J.Ahamed, H.H.Versteeg, M.Kerver, V.M.Chen, B.M.Mueller, P.J.Hogg, and W.Ruf (2006).
Disulfide isomerization switches tissue factor from coagulation to cell signaling.
  Proc Natl Acad Sci U S A, 103, 13932-13937.  
16757484 S.P.Bajaj, A.E.Schmidt, S.Agah, M.S.Bajaj, and K.Padmanabhan (2006).
High resolution structures of p-aminobenzamidine- and benzamidine-VIIa/soluble tissue factor: unpredicted conformation of the 192-193 peptide bond and mapping of Ca2+, Mg2+, Na+, and Zn2+ sites in factor VIIa.
  J Biol Chem, 281, 24873-24888.
PDB codes: 2a2q 2aer 2fir
15778956 D.Segal, and M.Eisenstein (2005).
The effect of resolution-dependent global shape modifications on rigid-body protein-protein docking.
  Proteins, 59, 580-591.  
15162493 A.Berchanski, B.Shapira, and M.Eisenstein (2004).
Hydrophobic complementarity in protein-protein docking.
  Proteins, 56, 130-142.  
15254262 A.Dorfleutner, E.Hintermann, T.Tarui, Y.Takada, and W.Ruf (2004).
Cross-talk of integrin alpha3beta1 and tissue factor in cell migration.
  Mol Biol Cell, 15, 4416-4425.  
15005335 J.H.Morrissey (2004).
Tissue factor: a key molecule in hemostatic and nonhemostatic systems.
  Int J Hematol, 79, 103-108.  
14722079 M.Huang, B.C.Furie, and B.Furie (2004).
Crystal structure of the calcium-stabilized human factor IX Gla domain bound to a conformation-specific anti-factor IX antibody.
  J Biol Chem, 279, 14338-14346.
PDB codes: 1nl0 3d69
14576054 R.Pawlinski, B.Pedersen, G.Schabbauer, M.Tencati, T.Holscher, W.Boisvert, P.Andrade-Gordon, R.D.Frank, and N.Mackman (2004).
Role of tissue factor and protease-activated receptors in a mouse model of endotoxemia.
  Blood, 103, 1342-1347.  
11790828 B.Ma, M.Shatsky, H.J.Wolfson, and R.Nussinov (2002).
Multiple diverse ligands binding at a single protein site: a matter of pre-existing populations.
  Protein Sci, 11, 184-197.  
12105230 K.E.Persson, B.O.Villoutreix, A.M.Thämlitz, K.E.Knobe, and J.Stenflo (2002).
The N-terminal epidermal growth factor-like domain of coagulation factor IX. Probing its functions in the activation of factor IX and factor X with a monoclonal antibody.
  J Biol Chem, 277, 35616-35624.  
11170384 D.Kirchhofer, C.Eigenbrot, M.T.Lipari, P.Moran, M.Peek, and R.F.Kelley (2001).
The tissue factor region that interacts with factor Xa in the activation of factor VII.
  Biochemistry, 40, 675-682.  
11080628 S.Monaco-Malbet, C.Berthet-Colominas, A.Novelli, N.Battaï, N.Piga, V.Cheynet, F.Mallet, and S.Cusack (2000).
Mutual conformational adaptations in antigen and antibody upon complex formation between an Fab and HIV-1 capsid protein p24.
  Structure, 8, 1069-1077.
PDB codes: 1e6j 1e6o
10828942 Y.Li, H.Li, S.J.Smith-Gill, and R.A.Mariuzza (2000).
Three-dimensional structures of the free and antigen-bound Fab from monoclonal antilysozyme antibody HyHEL-63(,).
  Biochemistry, 39, 6296-6309.
PDB codes: 1dqj 1dqm 1dqq
10451557 B.Selisko, A.F.Licea, B.Becerril, F.Zamudio, L.D.Possani, and E.Horjales (1999).
Antibody BCF2 against scorpion toxin Cn2 from Centuroides noxius Hoffmann: primary structure and three-dimensional model as free Fv fragment and complexed with its antigen.
  Proteins, 37, 130-143.  
10446191 J.Shobe, C.D.Dickinson, T.S.Edgington, and W.Ruf (1999).
Macromolecular substrate affinity for the tissue factor-factor VIIa complex is independent of scissile bond docking.
  J Biol Chem, 274, 24171-24175.  
10358763 K.C.Garcia, L.Teyton, and I.A.Wilson (1999).
Structural basis of T cell recognition.
  Annu Rev Immunol, 17, 369-397.  
9974392 O.Livnah, E.A.Stura, S.A.Middleton, D.L.Johnson, L.K.Jolliffe, and I.A.Wilson (1999).
Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation.
  Science, 283, 987-990.
PDB code: 1ern
  9605315 Y.A.Muller, R.F.Kelley, and Vos (1998).
Hinge bending within the cytokine receptor superfamily revealed by the 2.4 A crystal structure of the extracellular domain of rabbit tissue factor.
  Protein Sci, 7, 1106-1115.
PDB code: 1a21
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.