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Figure 1.
Fig. 1. Sequences of full-length and C-terminally truncated
rna1p/Rna1p derivatives from S. pombe/S. cerevisiae. Sequences
of the two yeast proteins (28, 29) were aligned with identical
residues being marked by vertical lines. The eight leucine-rich
repeats (residues 23-48, 85-112, 113-141, 179-206, 207-235,
236-264, 265-293, and 294-322, respectively, in rna1p) and the
two separating regions (residues 49-84 and 143-179,
respectively, in rna1p) are^ given separately. Amino acids
conserved in the majority of the^ repeats or the separating
regions are given in bold letters. Their positions are marked by
asterisks and by plus signs above the^ sequence of the
leucine-rich repeats and the separating regions, respectively.
The highly acidic C-terminal domain (residues 323-374^ in rna1p)
is marked by a dashed line above the sequence; the dashed^
double line close to the C terminus denotes a putative
amphipathic^  -helix.
Vertical lines identify the C-terminal residue of the^ different
truncation mutants of rna1p and Rna1p, respectively, that were
generated in this study. The vertical double arrow indicates a
site for chymotrypsin cleavage in wild-type rna1p that yields a
36.5-kDa fragment corresponding to the rna1p  330 mutant
derivative.
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