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Figure 7.
Figure 7. The hPar-6/PKCζ pathway is distinct from the
RafCAAX-mediated pathway. (a) Results of focus assays showing
the effect of PKCζ on the transforming synergy between Rac1 and
RafCAAX and on transformation induced by Ras. The amount of DNA
used in each transfection was: 100 ng for Rac1(G12V) or RafCAAX;
2 μg for KD-ζ or WT-ζ; 10 ng for Ras(G12V). RafCAAX-H
designates the higher amount of DNA (1 μg) that was used to
obtain a higher number of foci. Data are from two independent
experiments performed in duplicate. (b) Morphologies of
different foci transformed by the protein(s) indicated in each
panel.
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